Precementum- and / or cementum - derived chemotactic factor (CCTF) of tooth of mammalia, process for purifying the same, and drug for accelerating adhesion of new connective tissue, comprising the same as active ingredient

Abstract

A precementum- and/or cementum-derived chemotactic factor (CCTF) of a tooth of Mammalia, characterized in that a molecular weight measured by SDS-PAGE is 67000±1000. A process for purifying a precementum- and/or cementum-derived chemotactic factor (CCTF) of a tooth of Mammalia, wherein a molecular weight measured by SDS-PAGE is 67000±1000, which comprises collecting precementum and/or cementum from an extracted tooth of Mammalia and immersing them in saline or collagenase-containing saline with stirring to obtain an eluted ingredient, and purifying the eluted ingredient by molecular weight fractionation, ion-exchange adsorption chromatography and hydroxyapatite adsorption chromatography. A drug for accelerating adhesion of new connective tissue, comprising the precementum- and/or cementum-derived chemotactic factor (CCTF) as an active ingredient. [FIG. 1] Gel filtration chromatography P: Positive control (10% FBS-containing DMEM liquid culture medium) A: Negative control (liquid culture medium alone) Fraction A, fraction B [FIG. 2] DEAE-3SW ion-exchange chromatography P: Positive control: 10% FBS-containing DMEM liquid culture medium A: Negative control: DMEM liquid culture medium alone [FIG. 3] Hydroxyapatitie chromatography P: Positive control: 10% FBS-containing DMEM liquid culture medium A: Negative control: DMEM liquid culture medium alone [FIG. 4] [FIG. 5] Western blotting of CCTF using anti-BSP-II antibody [FIG. 6] Western blotting of CCTF using anti-BMP-2 antibody

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