Anti-GPE antibodies, their uses, and analytical methods for GPE
OwnerGregory Brian Thomas, Bernhard Hermann Heinrich Breier +1inventors
InventorsGregory Brian Thomas, Bernhard Hermann Heinrich Breier, David Charles Batchelor
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Abstract
GPE antibodies recognize GPE with high specificity. When used in a radioimmunoassay, they reliably measure the concentration of GPE. They may be used to extend the half-life of GPE both in vivo and in vitro, and in methods of purifying the GPE receptor. Two methods of rpHPLC accurately revolve GPE on the basis of its hydrophobicity.
Claims20
An anti-GPE antibody.
An antibody to Bolton and Hunter derivatized GPE.
An antibody to Bolton and Hunter derivatized GPE having the ability to specifically bind to Bolton and Hunter derivatized GPE at a final titer of 1:18,000 using Bolton and Hunter derivatized 125I-YGPE tracer.
Anti-GPE antiserum.
A reverse-phase HPLC method for the detection of GPE in a sample of body fluid or tissue, comprising:
- (a) derivatizing the sample with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate,
- (b) eluting the sample in a reverse phase HPLC system, and
- (c) detecting the GPE by excitation at 250 nm and detection at 395 nm.
A reverse-phase HPLC method for the detection of radioactive GPE in a sample of body fluid or tissue, comprising:
- (a) eluting the sample in a reverse phase HPLC system, and
- (b) detecting the GPE by detection of the emitted radioactivity.
Dependencies are inferred from the claim text.