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AssigneeCIS Bio International
InventorsMichel Mabile, Gerard Mathis, Etienne Jean-Pierre Jolu, Dominique Pouyat, Christophe Dumont
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The present invention relates to a method of measuring the luminescence emitted in a luminescent assay, which makes it possible to correct certain perturbations due to the measuring medium. Said method comprises employing at least one luminescent tracer compound and a luminescent compound used as an internal reference, which, when exposed to the same excitation wavelength, are capable of emitting at different wavelengths, λ2 and λ1 respectively, either by direct luminescence or by the induction of a luminescent emission, and correcting the measurement of the luminescence emitted by the tracer compound at wavelength λ2 on the basis of the measurement of the luminescence emitted by the reference compound at wavelength λl. The invention further relates to the use of said method in a homogeneous method of detecting and/or determining an analyte, and to a device for carrying it out.
A method of measuring the luminescence emitted in a luminescent assay, which comprises employing at least one luminescent tracer compound and a luminescent compound used as an internal reference, which, when exposed to the same excitation wavelength, are capable of emitting at different wavelengths, λ2 and λ1 respectively, either by direct luminescence or by the induction of a luminescent emission, and correcting the measurement of the luminescence emitted by the tracer compound at wavelength λ2 on the basis of the measurement of the luminescence emitted by the reference compound at wavelength λ1.
Dependencies are inferred from the claim text.