Back to results
US 2002/0177228 A1Published application
Method for producing preparations of mature and immature pancreatic endocrine cells, the cell preparation and its use for treatment of diabetes mellitus
AssigneeBeta-Cell N.V.
InventorDaniel Pipeleers
Life of this patent
Estimating the term…
Abstract
A method for preparing a preparation of mammalian pancreatic endocrine cells comprising the steps: dissociating intact pancreatic tissue into a cell suspension comprising single cells and cell aggregates; enriching said cell suspension with regard to the content in endocrine cells by separating single cells and cellular aggregates with size <100 μm; and removing contaminating non-endocrine cells by density centrifugation.
Claims22
A method for preparing a preparation of mammalian pancreatic endocrine cells comprising the steps of:
- i. dissociating intact pancreatic tissue into a cell suspension comprising single cells and cell aggregates;
- ii. enriching said cell suspension with regard to the content in endocrine cells by separating single cells and cellular aggregates with size <100 μm;
- and
- iii. removing contaminating non-endocrine cells by density centrifugation:
1 more element
Dependencies are inferred from the claim text.