Methods and compositions for crosslinking tissue
OwnerMathew F. Ogle, M. William Mirsch IIinventors
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Abstract
Tissue is crosslinked with compositions that include bridges. In some embodiments, the tissue includes linkers that bond to the tissue and bridges that connect two or more of the linkers. In other embodiments, bridges connect two or more modified sites in a modified tissue. The use of bridges in the crosslinking methods more readily forms crosslinks, particularly between different polypeptides in the tissue. The use of bridges can allow for greater modulation of the degree of crosslinking of a tissue.
Claims37
A tissue comprising linkers bonded to the tissue and a bridge molecule bonded between two or more of the linkers, wherein the linkers and the bridges are chemically different.
A method of crosslinking tissue comprising treating the tissue with a linker composition comprising linkers and a bridge composition comprising bridges wherein the linkers bond to the tissue and the bridges bond between two of the linkers, wherein the bridges and the linkers are chemically different.
A method of bonding two molecules of linkers comprising adding bridge molecules, wherein the bridge molecules bond between the two of the linkers.
A composition comprising linkers and bridge molecules wherein the bridge molecules are bonded between two of the linkers, wherein the bridges and the linkers are chemically different.
A tissue comprising bridge molecules, wherein the tissue is modified tissue and the bridge molecules are bonded to two or more modified sites in the modified tissue.
A method of crosslinking tissue comprising treating modified tissue with a bridge composition comprising bridge molecules wherein the bridges bond to two or more modified sites in the modified tissue.
Dependencies are inferred from the claim text.