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AssigneeIntrogen Therapeutics, Inc.
InventorsShuyuan Zhang, Capucine Thwin, Zheng Wu, Toohyon Cho
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The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production with cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.
A method for producing an adenovirus comprising:
The method of claim, 28, wherein said filtration is tangential flow filtration.
The method of claim, 28, wherein said filtration utilizes a 100 to 300K NMWC, regenerated cellulose, or polyether sulfone membrane.
An adenovirus produced according to a process comprising the steps of:
A method for the purification of an adenovirus comprising:
An adenovirus produced according to a process comprising the steps of:
A method for the purification of an adenovirus comprising:
An adenovirus produced according to a process comprising the steps of:
A 293 host cell adapted for growth in serum-free media.
Dependencies are inferred from the claim text.