Zace2: a human metalloenzyme
AssigneeZymoGenetics, Inc.
InventorsChristopher S. Piddington, Charles Petrie, Kimberly E. Shoemaker, Paul D. Bishop
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Abstract
Angiotensin-converting enzyme is a zinc metallopeptidase that plays roles in blood pressure regulation and fertility. The catalytic activities of angiotensin converting enzymes include the production of the potent vasopressor angiotensin II from angiotensin I, and the inactivation of the vasodilatory peptide bradykinin. Zace2 is a new form of human zinc metallopeptidase, characterized as an angiotensin converting enzyme paralog that is expressed primarily in tissues of the digestive system. Two murine orthologs are also described.
Claims20
An isolated polypeptide, comprising an amino acid sequence that is at least 70% identical to a reference amino acid sequence selected from the group consisting of:
- (a) amino acid residues 19 to 805 of a sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:6, and SEQ ID NO:9,
- (b) amino acid residues 19 to 738 of a sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:6, and SEQ ID NO:9,
- (c) amino acid residues 19 to 708 of a sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:6, and SEQ ID NO:9,
- (d) amino acid residues 19 to 613 of a sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:6, and SEQ ID NO:9,
- (e) amino acid residues 133 to 542 of a sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:6, and SEQ ID NO:9,
- (f) amino acid residues 344 to 542 of a sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:6, and SEQ ID NO:9, and
- (g) amino acid residues 371 to 402 of either SEQ ID NO:2 or SEQ ID NO:6,
- wherein the isolated polypeptide either (a) specifically binds with an antibody that specifically binds with a polypeptide consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:6, and SEQ ID NO:9, or (b) exhibits dipeptidyl carboxypeptidase activity.
An isolated nucleic acid molecule that encodes a Zace2 polypeptide, wherein the nucleic acid molecule is selected from the group consisting of (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:3, (b) a nucleic acid molecule encoding an amino acid sequence that comprises amino acid residues 19 to 738 of SEQ ID NO:2, and (c) a nucleic acid molecule that remains hybridized following stringent wash conditions to a nucleic acid molecule comprising the nucleotide sequence of nucleotides 89-2449 of SEQ ID NO:1, or the complement of nucleotides 89-2449 of SEQ ID NO:1.
An isolated nucleic acid molecule that encodes a Zace2 polypeptide, wherein the nucleic acid molecule is selected from the group consisting of (a) a nucleic acid molecule encoding an amino acid sequence that comprises amino acid residues 19 to 738 of SEQ ID NO:6, (b) a nucleic acid molecule encoding an amino acid sequence that comprises amino acid residues 19 to 738 of SEQ ID NO:9, and (c) a nucleic acid molecule that remains hybridized following stringent wash conditions to a nucleic acid molecule comprising the nucleotide sequence of nucleotides 106-2520 of SEQ ID NO:5, or the complement of nucleotides 106-2520 of SEQ ID NO:5.
Dependencies are inferred from the claim text.