Novel postassium channel protein
AssigneeYamanouchi Pharmaceutical Co., Ltd.
InventorsAkira Miyake, Shinobu Mochizuki, Hiromichi Yokoi
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AssigneeYamanouchi Pharmaceutical Co., Ltd.
InventorsAkira Miyake, Shinobu Mochizuki, Hiromichi Yokoi
Estimating the term…
The present invention relates to a novel potassium channel protein expressed exclusively in the brain, a gene encoding this protein, a vector containing this gene, a host cell containing this vector, and so on. A typical method for obtaining the potassium channel protein of the present invention; mRNA is extracted from human cells or tissue capable of producing the potassium channel protein of the present invention. Then use is made of two primers between which the channel protein mRNA or a part of the mRNA region is located by using the thus extracted mRNA as a template. By carrying out a reverse transcriptase-polymerase chain reaction (to be referred to as RT-PCR hereinafter), the channel protein cDNA or a part thereof can be obtained. Thereafter, the channel protein can be produced by integrating the thus obtained novel potassium channel cDNA or a part of the same into an appropriate expression vector and then effecting its expression in a host cell.
A potassium channel protein or an equivalent thereof, which has an amino acid sequence selected from either of Sequence Nos. 2 and 6, or an amino acid sequence resulting from said amino acid sequence by substitution, deletion or insertion of certain amino acid(s), and is expressed exclusively in the brain.
A potassium channel protein which has an amino acid sequence selected from either of Sequence Nos. 2 and 6.
A gene which has a gene sequence encoding an amino acid sequence selected from either of Sequence Nos. 2 and 6.
A gene which has a gene sequence selected from either of the 6th to 3257th gene sequence of Sequence No. 1 or the 4th to 3057th gene sequence of Sequence No. 5, or a gene which is degenerate with respect to said gene.
Dependencies are inferred from the claim text.