Moraxella catarrahalis outer membrane protein-106 polypeptide, gene sequence and uses thereof
AssigneeAntex Biologics Inc.
InventorsKenneth Tucker, Laura Plosila
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Abstract
The invention discloses the Moraxella catarrhalis outer membrane protein-106 (OMP106) polypeptide, polypeptides derived therefrom (OMP106-derived polypeptides), nucleotide sequences encoding said polypeptides, and antibodies that specifically bind the OMP106 polypeptide and/or OMP106-derived polypeptides. Also disclosed are immunogenic, prophylactic or therapeutic compositions, including vaccines, comprising OMP106 polypeptide and/or OMP106-derived polypeptides. The invention additionally discloses methods of inducing immune responses to M. catarrhalis and M. catarrhalis OMP106 polypeptides and OMP106-derived polypeptides in animals.
Claims41
An isolated or substantially pure OMP106 polypeptide, which is an outer membrane polypeptide of Moraxella catarrhalis, and has a molecular weight of about 180 kD to about 230 kD as determined in SDS polyacrylamide gel electrophoresis using rabbit skeletal muscle myosin and E. coli β-galactosidase as the 200 kD and 116.25 kD molecular weight standards, respectively.
An isolated or substantially pure OMP106 polypeptide comprising a sequence substantially homologous to the sequence of SEQ ID NO:1.
A substantially pure DNA comprising a nucleotide sequence encoding the peptide of SEQ ID NO:1.
A substantially pure DNA encoding an OMP106 polypeptide, which comprises a nucleotide sequence that hybridizes under high stringency conditions to the sequence of SEQ ID NO:4 or the complement of sequence of SEQ ID NO:4.
A method of producing a non-hemagglutinating cultivar of M. catarrhalis from a HA M. catarrhalis strain or cultivar, which comprises serially passaging a HA M. catarrhalis strain or cultivar in static liquid cultures.
Plasmid pOMP106X obtainable from E. coli Top10 (pOMP106X), as deposited with the ATCC and assigned accession number 98579.
A substantially pure DNA encoding an OMP106 polypeptide, which comprises a nucleotide sequence that hybridizes to the sequence of SEQ ID NO:9 or the complement of sequence of SEQ ID NO:9.
An isolated or substantially pure OMP106 polypeptide comprising a sequence substantially homologous to the sequence of SEQ ID NO:10.
Dependencies are inferred from the claim text.