Method for producing ascorbic acid intermediates
OwnerMathew Grant Boston, Barbara A. Swansoninventors
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Abstract
The present invention relates to non-fermentative methods for the production of ASA intermediates, KDG, DKG and KLG and methods for the regeneration of co-factor. The invention provides genetically engineered host cells comprising heterologous nucleic acid encoding enzymes useful in the process.
Claims62
A process for the non-fermentative production of KDG or DKG from a carbon source comprising, enzymatically oxidizing the carbon source by at least one oxidative enzymatic activity to yield KDG or DKG.
A process for the non-fermentative production of 2-KLG from a carbon source, comprising the following steps in any order, enzymatically oxidizing the carbon source by at least one oxidative enzymatic activity to an oxidation product;
- and enzymatically reducing said oxidation product by at least one reducing enzymatic activity to 2-KLG.
A host cell comprising nucleic acid having a mutation in the gene encoding GHD activity.
A host cell comprising nucleic acid having a mutation in the gene encoding KDGDH activity.
Dependencies are inferred from the claim text.