Steroid hormone binding protein
AssigneeChugai Seiyaku Kabushiki Kaisha, a Japanese corporation
InventorYuichi Hirata
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Abstract
Several ESTs deduced as a part of a cDNA encoding a protein that is homologous with a membrane-bound steroid binding protein PMBP were found. Based on the sequence data of these ESTs, a consensus sequence was extracted, and primers were designed based on this consensus sequence. Using the thus designed primers, a polymerase chain reaction of human genes was effected. As a result, a gene encoding a novel steroid hormone binding protein that is homologous with PMBP was successfully isolated from a human for the first time.
Claims29
A substantially pure polypeptide comprising an amino acid sequence at least 60% identical to SEQ ID NO:4, wherein the polypeptide specifically binds to a steroid.
A substantially pure polypeptide comprising the amino acid sequence of SEQ ID NO:4.
A substantially pure polypeptide comprising the amino acid sequence of SEQ ID NO:4, with up to 30 conservative amino acid substitutions, wherein the polypeptide specifically binds a steroid.
A substantially pure polypeptide encoded by a nucleic acid that hybridizes under high stringency conditions to a probe the sequence of which consists of SEQ ID NO:3, wherein the polypeptide specifically binds a steroid.
An isolated nucleic acid comprising a strand that hybridizes under high stringency conditions to a single stranded probe, the sequence of which consists of SEQ ID NO:3, or the complement of SEQ ID NO:3.
A method of screening for a compound that binds to a polypeptide, the method comprising
- providing a polypeptide comprising an amino acid sequence at least 60% identical to SEQ ID NO:4;
- contacting a test compound with the polypeptide;
- and
- determining whether the test compound has bound to the polypeptide.
Dependencies are inferred from the claim text.