Novel fluorescent proteins
AssigneeBioImage A/S
InventorsSara Petersen Bjorn, Len Pagliaro, Ole Thastrup
Life of this patent
Estimating the term…
Abstract
A GFP with an F64L mutation and an E222G mutation is provided. This GFP has a bigger Stokes shift compared to other GFPs making it very suitable for high throughput screening due to a better resolution. This GFP also has an excitation maximum between the yellow GFP and the cyan GFP allowing for cleaner band separation when used together with those GFPs.
Claims19
A fluorescent protein derived from Green Fluorescent Protein (GFP) or any functional GFP analogue, wherein the amino acid in position 1 preceding the chromophore has been mutated and wherein the Glutamic acid in position 222 has been mutated said mutated GFP has an excitation maximum at a higher wavelength and the fluorescence is increased when the mutated GFP is expressed in cells incubated at a temperature of 30° C. or above compared to wild-type GFP.
Dependencies are inferred from the claim text.