Compositions and methods for detection of ehrlichia canis and ehrlichia chaffeensis antibodies
AssigneeIDEXX Laboratories, Inc.
InventorsRobert Lawton, Thomas Patrick O'Connor JR., Barbara Ann Bartol, Paul Scott MacHenry
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Abstract
The invention provides methods and compositions for the detection of Ehrlichia canis and Ehrlichia chaffeensis antibodies and antibody fragments.
Claims34
A composition of matter comprising an isolated polypeptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 and variants thereof.
A method of detecting presence of antibodies to Ehrlichia comprising:
- (a) contacting one or more polypeptides selected from the group consisting of the polypeptides shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, and variants thereof, with a test sample suspected of comprising antibodies to Ehrlichia, under conditions that allow polypeptide/antibody complexes to form;
- (b) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that antibodies to Ehrlichia are present in the test sample.
A device containing one or more polypeptides selected from the group consisting of the polypeptides shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, and variants thereof.
An article of manufacture comprising packaging material and, contained within the packaging material, one or more polypeptides selected from the group consisting of the polypeptides shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, and variants thereof.
A method of diagnosing an Ehrlichia infection in a mammal comprising:
- (a) obtaining a biological sample from a mammal suspected of having an Ehrlichia infection;
- (b) contacting one or more polypeptides selected from the group consisting of the polypeptides shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, and variants thereof, with the biological sample under conditions that allow polypeptide/antibody complexes to form;
- (c) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that the mammal has an Ehrlichia infection.
A monoclonal antibody that specifically binds to at least one epitope of an Ehrlichia canis or Ehrlichia chaffeensis polypeptide, said polypeptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7.
Dependencies are inferred from the claim text.