Novel antibody composition for isolating human cells from human-murine chimeric hematopoietic cell suspensions
AssigneeStemCell Technologies Inc.
InventorsTerry E. Thomas, Connie J. Eaves
Life of this patent
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Abstract
The present invention relates to an antibody composition which contains antibodies directed to murine leukocyte and murine erythroid cells. This composition is used in a novel negative selection process to enrich for human hematopoietic cells from a sample from human-murine chimeric mice. The invention also relates to kits for carrying out this process.
Claims14
A negative selection process for enriching and recovering human cells in a sample containing human cells and murine cells comprising:
- (a) reacting the sample with an antibody composition containing antibodies capable of binding to murine leukocytes under conditions such that conjugates are formed between the antibodies and murine leukocytes;
- (b) removing the conjugates;
- and
- (c) recovering a cell population which is enriched in human cells and depleted of murine leukocytes.
An antibody composition comprising an antibody specific for murine leukocytes and an antibody specific for murein erythroid cells.
Dependencies are inferred from the claim text.