In vitro methods for determining in vivo thrombotic events
AssigneeThe Scripps Research Institute
InventorsMark H. Ginsberg, Andrew L. Frelinger III, Edward F. Plow
Life of this patent
Estimating the term…
Abstract
Diagnostic systems, methods, polypeptides and antibodies for detecting the presence of C-terminal hGPIIb fragment of the platelet receptor GPIIb-IIIa in a body fluid sample are disclosed.
Claims26
A substantially isolated C-terminal hGPIIb fragment that has a molecular weight of about 3,900 daltons, comprising an amino acid residue sequence that corresponds to the carboxy terminal portion of the amino acid residue sequence of hGPIIb shown in SEQ ID NO 1, including the amino acid residue sequence represented by the formula
- -IHPAHHK-,
- said fragment having the capacity to immunoreact with the monoclonal antibody, PMI-1.
A hGPIIb analog comprising a polypeptide of no more than about 200 residues, said polypeptide having an amino acid residue sequence that corresponds to the sequence of hGPIIb shown in SEQ ID NO 1 and includes at least about 7 contiguous amino acid residues from the amino-acid residue sequence of hGPIIb shown in SEQ ID NO 1 from residue 173 to residue 200.
An antibody comprising antibody molecules that immunoreact with a polypeptide having an amino acid residue sequence as shown in SEQ ID NO 1 from residue 173 to residue 193, and do not substantially immunoreact with a polypeptide represented by the formula REQNSLDSWGPK having the amino acid residue sequence shown in SEQ ID NO 1 from residue 113 to residue 124.
A method for detecting the presence of C-terminal hGPIIb fragment in a body fluid sample containing cells, comprising the steps of:
- a) separating the cells from said body fluid sample to form a cell-free body fluid sample;
- b) admixing an aliquot of said cell-free body fluid sample with a composition that contains an antibody that:
- i) immunoreacts with said C-terminal hGPIIb fragment that includes an amino acid residue sequence as shown in SEQ ID NO 1 from residue 173 to residue 200, and
- ii) does not substantially immunoreact with a polypeptide represented by the formula REQNSLDSWGPK having the amino acid residue sequence shown in SEQ ID NO 1 from residue 113 to residue 124, to form an immunoreaction admixture;
- c) maintaining said immunoreaction admixture under biological assay conditions for a time period sufficient to form an immunoreaction product;
- and
- d) detecting the presence of said immunoreaction product, and thereby the presence of said fragment.
A diagnostic system in kit form for assaying for the presence of C-terminal hGPIIb fragment in a body fluid sample, comprising a package containing, in an amount sufficient to perform at least one assay, an antibody composition comprising antibody molecules that:
- a) immunoreact with a polypeptide having an amino acid residue sequence as shown in SEQ ID NO 1 from residue 173 to residue 193, and
- b) do not substantially immunoreact with a polypeptide represented by the formula REQNSLDSWGPK having the amino acid residue sequence shown in SEQ ID NO 1 from residue 113 to residue 124.
Dependencies are inferred from the claim text.