Stimulable phosphor sheet for detection of substances originating from living body or its analogues
AssigneeFuji Photo Film Co., Ltd.
InventorYuichi Hosoi
Life of this patent
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Abstract
A method employing a porous material sheet which has at predetermined positions plural dots each being composed of a group of probe molecules and a stimulable phosphor sheet having a phosphor layer which contains a stimulable phosphor in an amount of 10 to 140 g/m2 is favorably utilized to detect by autoradiography radioactively labeled substances originating from living body or its analogues which are able to be fixed to the probe molecules by biochemically specific binding reaction.
Claims19
A method employing a porous material sheet which has at predetermined positions plural dots each comprising a group of probe molecules and a stimulable phosphor sheet having a phosphor layer which contains a stimulable phosphor in an amount of 10 to 140 g/m2, to detect by autoradiography radioactively labeled substances originating from living body or its analogues which are able to be fixed to the probe molecules by biochemically specific binding reaction.
A laminate of a stimulable phosphor sheet having a phosphor layer which contains a stimulable phosphor in an amount of 10 to 140 g/m2 and a porous material sheet which has at predetermined positions plural dots each comprising a group of probe molecules and radioactively labeled substances originating from living body or its analogues which are fixed to the probe molecules by biochemically specific binding reaction.
A process for detecting complementary nucleic acid fragments which comprises the steps of:
- preparing a porous material sheet which has at predetermined positions plural dots each comprising a group of probe molecules;
- bringing sample substances originating from living body or its analogues having a radioactive label in a liquid phase into contact with the prepared porous material sheet, whereby target substances in the sample substances are fixed to the probe molecules by biochemically specific reaction;
- removing unfixed sample substances from the porous material sheet;
- keeping the last-mentioned porous material sheet in contact with a stimulable phosphor sheet having a phosphor layer which contains a stimulable phosphor in an amount of 10 to 140 g/m2, whereby the stimulable phosphor layer absorbs and stores radiation energy of the radioactive label coming from the fixed target substances;
- irradiating the stimulable phosphor sheet with a stimulating light, whereby the phosphor layer in which the radiation energy is stored releases stimulated emissions;
- detecting the stimulated emissions photoelectrically to obtain a series of electric signals;
- and
- processing the electric signals to detect dots in which the target substances are present.
A process for detecting complementary nucleic acid fragments which comprises the steps of:
- preparing a composite material sheet which comprises partitions two-dimensionally extending on a sheet plane to form on the sheet plane plural fine sections surrounded by the partitions and porous material portions each of which is placed in the fine section, to each porous material portion of which a group of probe molecules are attached;
- bringing sample substances originating from living body or its analogues having a radioactive label in a liquid phase into contact with the prepared composite material sheet, whereby target substances in the sample substances are fixed to the probe molecules by biochemically specific reaction;
- removing unfixed sample substances from the composite material sheet;
- keeping the last-mentioned composite material sheet in contact with a stimulable phosphor sheet having a phosphor layer which contains a stimulable phosphor in an amount of 10 to 140 g/m2, whereby the stimulable phosphor layer absorbs and stores radiation energy of the radioactive label coming from the fixed target substances;
- irradiating the stimulable phosphor sheet with a stimulating light, whereby the phosphor layer in which the radiation energy is stored releases stimulated emissions;
- detecting the stimulated emissions photoelectrically to obtain a series of electric signals;
- and
- processing the electric signals to detect dots in which the target substances are present.
Dependencies are inferred from the claim text.