Mdr1 variants and methods for their use
AssigneeWashington State University Research Foundation
InventorsKatrina L. Mealey, Steven A. Bentjen
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Abstract
This invention provides the identification of a truncation polymorphism of the mdr1 gene that is linked to ivermectin sensitivity in subjects, such as collies. Also provided are methods for detecting drug transport sensitivity in a subject, and animal models and in vitro cell systems using cells from animals having an mdr1 truncation.
Claims42
A method of detecting ivermectin sensitivity in a subject, comprising determining whether a gene-truncation mutation in a mdr1-encoding sequence of the subject or a truncated P-pg is present in the subject, wherein presence of the gene-truncation mutation or truncation of P-gp indicates that the subject is sensitive to ivermectin.
A method of making a treatment decision for a subject, comprising determining whether a gene-truncation mutation in a mdr1-encoding sequence of the subject or a truncated P-pg is present in the subject, wherein presence of the gene-truncation mutation or truncation of P-gp influences the treatment decision.
A kit for use in diagnosing or detecting ivermectin sensitivity in a subject, comprising a probe that specifically hybridizes to an mdr1 gene-truncation mutation associated with ivermectin sensitivity.
A kit for use in diagnosing ivermectin sensitivity in a subject, comprising a P-gp-specific binding agent.
An oligonucleotide that specifically hybridizes to a canine mdr1 gene-truncation mutation.
A method of determining a P-gp influenced biological effect of a compound on a canine cellular system, comprising:
- contacting a canine cell with the compound, wherein the cell has a truncation mutation in its mdr1 gene, and
- comparing a characteristic of the canine cell contacted with the compound with the characteristic of a similar canine cell not contacted with the compound, wherein a difference in the characteristic between the two cells is indicative of the P-gp influenced biological effect.
An animal model useful for studying a P-gp influenced biological effect of a compound, comprising a Collie identified as being homozygous or heterozygous for a truncation mutation in the mdr1 gene.
Dependencies are inferred from the claim text.