Non-alloying core shell nanoparticles
AssigneeNanosphere, Inc.
InventorsChad A. Mirkin, Yun-Wei Cao, Rongchao Jin
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Abstract
The present invention relates composite core/shell nanoparticles and a two-step method for their preparation. The present invention further relates to biomolecule-core/shell nanoparticle conjugates and methods for their preparation. The invention also relates to methods of detection of biomolecules comprising the biomolecule or specific binding substance-core/shell nanoparticle conjugates.
Claims35
An core/shell nanoparticle comprising:
- (a) an inner metal-containing nanoparticle core;
- and
- (b) an outer non-alloying gold shell surrounding the nanoparticle core.
A core/shell nanoparticle specific binding substance conjugate comprising:
- (a) an inner metal-containing nanoparticle core;
- (b) an outer non-alloying gold shell surrounding the nanoparticle core;
- and
- (c) specific binding substance attached to the gold shell.
A core/shell nanoparticle oligonucleotide conjugate comprising:
- (a) an inner metal-containing nanoparticle core;
- (b) an outer non-alloying gold shell surrounding the nanoparticle core;
- and
- (c) oligonucleotides attached to the gold shell.
A silver/gold core/shell nanoparticle comprising:
- (a) an inner silver nanoparticle core;
- and
- (b) an outer non-alloying gold shell surrounding the nanoparticle core.
A magnetic core/shell nanoparticle comprising:
- (a) an inner Fe3O4 nanoparticle core;
- and
- (b) an outer non-alloying gold shell surrounding the nanoparticle core.
A method for preparing core/shell nanoparticles comprising the steps of:
- (a) providing inner metal-containing nanoparticle cores;
- (b) treating the inner metal-containing nanoparticle cores simultaneously with a solution comprising a gold salt and a solution comprising a reducing silverent at 0° C. to produce a non-alloying gold shell surrounding the nanoparticle cores;
- and
- (c) isolating the core/shell nanoparticles.
A product produced by the method of 24.
A method of detecting a target analyte bound to a surface comprising:
- (a) providing a surface that includes a bound target analyte;
- (b) contacting the surface with a solution comprising core/shell nanoparticle receptor conjugate, wherein the receptor specifically binds to the analyte, wherein the contacting takes place under conditions effective to allow binding of the nanoparticle conjugates with the bound nucleic acid;
- (c) subjecting the nanoparticle conjugate to an external magnetic field so as to accelerate movement of the nanoparticle conjugate to the surface to promote binding interaction between the nanoparticle conjugate and the target analyte;
- (c) removing from the surface any nanoparticle conjugates that have not bound with the target analyte;
- and
- (d) observing a detectable change brought about by binding interaction of the target analyte with the nanoparticle conjugates.
Dependencies are inferred from the claim text.