Compositions and methods for detecting human immunodeficiency virus 2 (HIV-2)
AssigneeGen-Probe Incorporated
InventorsYeasing Y. Yang, Terrie A. Burrell
Life of this patent
Estimating the term…
Abstract
Compositions and methods for synthesizing and detecting HIV-2 specific amplicons. Particularly described are oligonucleotides that are useful as hybridization probes, and amplification primers that facilitate detection of very low levels of HIV-2 nucleic acids.
Claims56
A composition for detecting an HIV-2 nucleic acid sequence, comprising:
- a first amplification oligonucleotide comprising a sequence of 9-34 contiguous bases contained within the sequence of SEQ ID NO:9, said first amplification oligonucleotide having a length of up to 100 nucleotides;
- and
- a second amplification oligonucleotide comprising a sequence of 19-40 contiguous bases from the sequence of SEQ ID NO:
- 1, said second amplification oligonucleotide having a length of up to 100 nucleotides.
A method for determining whether a biological sample comprising nucleic acids includes an HIV-2 nucleotide base sequence, said method comprising the steps of:
- contacting the nucleic acids of the biological sample with a composition comprising,
- a first amplification oligonucleotide comprising a sequence of 9-34 contiguous bases contained within the sequence of SEQ ID NO:9, said first amplification oligonucleotide having a length of up to 100 nucleotides, and
- a second amplification oligonucleotide comprising a sequence of 19-40 contiguous bases from the sequence of SEQ ID NO:1, said second amplification oligonucleotide having a length of up to 100 nucleotides;
- amplifying any of said HIV-2 nucleotide base sequence present in said biological sample to produce amplified nucleic acids;
- and
- detecting said amplified nucleic acids produced in the amplifying step, whereby detection of said amplified nucleic acids indicates that said biological sample included the HIV-2 nucleotide base sequence.
An oligonucleotide comprising the sequence of SEQ ID NO:21 or the complement thereof and a detectable label, said oligonucleotide having a length of up to 35 nucleotides.
A method for detecting the presence of HIV-2 nucleic acids in a biological sample, comprising the steps of:
- (a) providing to said biological sample a hybridization probe comprising the sequence of SEQ ID NO:21 or the complement thereof and a detectable label, said oligonucleotide having a length of up to 35 nucleotides;
- (b) hybridizing under a high stringency condition any HIV-2 nucleic acid that may be present in the biological sample with said hybridization probe to form a probe:target duplex;
- and
- (c) detecting said probe:target duplex as an indicator of the presence of HIV-2 in the biological sample.
A kit for detecting HIV-2 nucleic acids, comprising:
- (a) a first amplification oligonucleotide comprising a sequence of 9-34 contiguous bases contained within the sequence of SEQ ID NO:9, said first amplification oligonucleotide having a length of up to 100 nucleotides;
- and
- (b) a second amplification oligonucleotide comprising a sequence of 19-40 contiguous bases from the sequence of SEQ ID NO:
- 1, said second amplification oligonucleotide having a length of up to 100 nucleotides.
Dependencies are inferred from the claim text.