Hepatitis B virus surface antigen mutant and methods of detection thereof
AssigneeAbbott Laboratories
InventorsPaul F. Coleman, Isa K. Mushahwar
Life of this patent
Estimating the term…
Abstract
The subject invention relates to a novel hepatitis B surface antigen mutant and methods of detecting this mutant, and/or antibodies thereto, in patient samples. In particular, the mutant contains a substitution of amino acid threonine for the amino acid alanine at position 123 in the amino acid sequence of the hepatitis B surface antigen (HBsAg) protein.
Claims26
An isolated nucleotide sequence having at least 70% identity to SEQ ID NO:
- 1 or to a fragment of said sequence which specifically hybridizes to the complement of SEQ ID NO:
- 1.
An isolated nucleotide sequence comprising a sequence encoding a mutant hepatitis B surface antigen (HBsAg) “a” determinant in which the mutation, as compared to the wildtype sequence, is the substitution of the amino acid Ala for the amino acid Thr at position 123 of the HBsAg sequence.
A purified polypeptide having at least 70% identity to SEQ ID NO:
- 2.
An antibody which binds to a mutant HBsAg “a” determinant and does not cross-react with the native HBsAg “a” determinant, wherein the mutation of said mutant “a” determinant, as compared to the wildtype sequence, is the substitution of the amino acid Ala for the amino acid Thr at position 123 of the HBsAg sequence.
An isolated mutant hepatitis B virus, wherein said virus has a modified HBsAg “a” determinant comprising a substitution of the amino acid Ala for the amino acid Thr at position 123 of the HBsAg sequence.
A polynucleotide probe comprising a Hepatitis B Virus genomic sequence encoding a modified HBsAg “a” determinant, wherein said modified HBsAg “a” determinant results from substitution of alanine for guanine at position 561 of the nucleotide sequence of said Hepatitis B Virus.
An isolated nucleotide sequence having at least 70% identity to SEQ ID NO:
- 4 or to a fragment of said sequence which specifically hybridizes to the complement of SEQ ID NO:
- 4.
A purified polypeptide having at least 70% identity to SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.