Novel diagnostic screens for type 1 diabetes (IDDM)
AssigneeTrustees of Boston University
InventorsBarbara E. Corkey, Nicholas R. Husni
Life of this patent
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Abstract
The present invention features methods for diagnosing insulin-dependent diabetes mellitus (IDDM) in a test subject, for example a subject at risk of developing IDDM, which involve detecting hyper-responsive Ca2+ mobilization in cells obtained from the test subject. Hyper-responsive Ca2+ mobilization can be detected in cells contacted with a stimulatory agent, preferably in cells contacted with a potentiating agent and a stimulatory agent. Methods of the present invention include comparing Ca2+ mobilization in cells from the test subject to Ca2+ mobilization in cells from a control subject. The present invention further features kits for use in the detection of IDDM.
Claims20
A method for diagnosing IDDM in a test subject comprising detecting hyper-responsive Ca2+ mobilization in cells obtained from the test subject.
A method of identifying a subject at risk of developing IDDM comprising comparing Ca2+ mobilization in a test cell sample obtained from the subject to Ca2+ mobilization in a control cell sample and identifying the subject at risk by detecting a difference in Ca2+ mobilization in the test cell sample as compared to the control cell sample.
A kit comprising a control cell sample, a stimulatory agent, a potentiating agent and instructions for use.
Dependencies are inferred from the claim text.