Process for the fermentative preparation of D-pantothenic acid and/or salts thereof
AssigneeDegussa AG
InventorsNicole Dusch, Achim Marx, Walter Pfefferle, Georg Thierbach
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Abstract
The invention provides a process for the fermentative preparation of D-pantothenic acid and/or salts thereof or feedstuffs additives comprising these by fermentation of coryneform bacteria, in particular those which already produce D-pantothenic acid, wherein the nucleotide sequence(s) in the coryneform bacteria which code(s) for the glyA gene is (are) enhanced, in particular over-expressed.
Claims15
Process for the preparation of D-pantothenic acid and/or salts thereof or feedstuffs additives comprising these by fermentation of coryneform bacteria, in particular those which already produce D-pantothenic acid, in which
- a) the nucleotide sequence(s) in the bacteria which code(s) for the endogenous glyA gene is (are) enhanced, in particular over-expressed, under conditions which are suitable for the production of serine hydroxymethyl transferase,
- b) the D-pantothenic acid and/or salts thereof in the medium or in the cells of the microorganisms are concentrated, and
- c) after conclusion of the fermentation, the desired products are isolated, the biomass and/or optionally further constituents of the fermentation broth being separated off in an amount of ≧0 to 100%,
- D-pantothenic acid being produced by the microorganisms.
A vector for expression of the glyA gene of C. glutamicum, which comprises a promoter and the gene sequence.
Coryneform bacteria, in particular of the genus Corynebacterium, transformed with the plasmid pVWEx2glyA, shown in FIG. 1.
Dependencies are inferred from the claim text.