DNA vaccines against poxviruses
AssigneeThe United States of America as represented by the Secretary of the Army
InventorsJay W. Hooper, Alan L. Schmaljohn, Connie S. Schmaljohn
Life of this patent
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Abstract
In this application is described a poxvirus naked DNA vaccine which protects animals against poxvirus challenge comprising IMV and EEV nucleic acids from poxvirus. Methods of use of the vaccine and its advantages are described.
Claims16
A DNA vaccine against poxviruses comprising at least one nucleic acids encoding an intracellular mature virion antigen and at least one nucleic acid encoding an extracellular enveloped virion antigen of a poxvirus.
A method for inducing in a subject an immune response against poxvirus infection comprising administering to said subject an immunologically effective amount of at least one nucleic acid encoding an intracellular mature virion antigen and at least one nucleic acid encoding an extracellular mature virion antigen of said poxvirus in an acceptable diluent.
A composition of matter comprising a carrier particle;
- and a DNA sequence coated onto the carrier particle, the DNA sequence comprising a promoter operative in the cells of a mammal and a protein coding region coding for a poxvirus antigen chosen from the group consisting of:
- IMV or EEV antigen.
A method for inducing a protective immune response to a poxvirus in a mammal, comprising
- (i) preparing a nucleic acid encoding an antigen of poxvirus operatively linked to a promoter operative in cells of a mammal;
- (ii) coating the nucleic acid in (i) onto carrier particles;
- (iii) accelerating the coated carrier particles into epidermal cells of the mammal in vivo;
- and
- (iv) detecting a protective immune response in said mammal upon exposure to a poxvirus.
A multivalent vaccine for protection against infection with more than one poxvirus comprising a composition of matter comprising a carrier particle having one or more DNA sequence coated onto the carrier particle, the DNA sequence comprising a promoter operative in the cells of a mammal and a nucleic acid coding for an antigen, said antigen selected from the group consisting of an IMV antigen and an EEV antigen, of a first poxvirus said poxvirus selected from the group consisting of Orthopoxvirus, Parapoxvirus, Caripoxvirus, Suipoxvirus, Leporipoxvirus, Avipoxvirus, Yatapoxvirus, Molluscipoxvirus, macropod poxvirus, and crocodilian poxvirus.
Dependencies are inferred from the claim text.