Isolation and characterization of the csa operon (ETEC-CS4 pili) and methods of using same
AssigneeUniversity of Maryland, Baltimore
InventorsZeev Altboum, Myron M. Levine, Eileen M. Barry
Life of this patent
Estimating the term…
Abstract
Compositions comprising products of the csa operon, an isolated nucleic acid encoding the csa operon or functional fragments thereof, purified polypeptide products of the csa operon or functional fragments thereof, methods of eliciting an immune response to these products, and methods of producing products of the csa operon are disclosed herein.
Claims81
An immunogenic composition comprising:
- a recombinant product of a csa operon and a carrier.
An isolated nucleotide sequence comprising a csa operon or a functional fragment thereof.
An expression vector comprising a csa operon nucleotide sequence or an antigenic fragment thereof.
A purified polypeptide sequence expressed from a recombinant csa operon or an antigenic fragment thereof.
A method of generating an immune response, comprising:
- providing an immunogenic composition to a subject, wherein said immunogenic composition comprises a recombinant product of a csa operon;
- and
- contacting said subject with said immunogenic composition, whereby an immune response is generated in said subject.
A method of producing a polypeptide product from a csa operon or functional fragment thereof, comprising:
- providing the csa operon in an expression vector;
- introducing the expression vector into a host cell, such that a recombinant host cell is produced;
- and
- subjecting to the recombinant host cell to conditions such that a protein from the csa operon is expressed.
A method for generating an immune response in a vertebrate against ETEC, comprising administering to the vertebrate an amount of a polynucleotide operatively encoding at least an immunogenic portion of the csa operon and having at least about 15 nucleotides, or administering a polypeptide encoded thereby.
Dependencies are inferred from the claim text.