Isolated nucleic acid molecules coding for tumor rejection antigen precursors of members of the MAGE-C and MAGE-B families and uses thereof
OwnerSophie Lucas, Thierry Boon-Falleurinventors
Life of this patent
Estimating the term…
Abstract
The invention relates to isolated nucleic acid molecules which code for antigens expressed by tumor cells which maybe recognized by cytotoxic T cells, leading to lysis of the tumor cells which express it. This invention also relates to vectors which are designed to encode the antigen expressed by tumor cells and also to cells transfected by the nucleic acid molecules or vectors which comprise the nucleic acid molecules. Various therapeutic and diagnostic uses arising out of the properties of the nucleic acid molecules and the antigens for which these code are also part of this invention.
Claims33
An isolated nucleic acid molecule which encodes a tumor rejection antigen precursor “TRAP” having an amino acid sequence of a TRAP encoded by a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 21, SEQ ID NO:
- 23 and SEQ ID NO:
- 25.
An isolated nucleic acid molecule having a nucleotide sequence which encodes a tumor rejection antigen precursor, said isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 21, SEQ ID NO:
- 23 and SEQ ID NO:
- 25.
A polytope comprising a plurality of tumor rejection antigens wherein said antigens are derived from a tumor rejection antigen precursor selected from the group consisting of MAGE-C3, MAGE-B5, and MAGE-B6 tumor rejection antigen precursors.
Kit useful in a polymerase chain reaction based assay, comprising an oligonucleotide having a sequence of nucleotides 175-195 of SEQ ID NO:
- 21 and an oligonucleotide having a nucleotide sequence that is complementary to nucleotides 711-731 of SEQ ID NO:
- 21.
Kit useful in a polymerase chain reaction based assay, comprising an oligonucleotide having a sequence of nucleotides 370-394 of SEQ ID NO:
- 23 and an oligonucleotide having a nucleotide sequence that is complementary to nucleotides 682-705 of SEQ ID NO:
- 23.
Kit useful in a polymerase chain reaction based assay, comprising an oligonucleotide having a sequence of nucleotides 114-137 of SEQ ID NO:
- 25 and an oligonucleotide having a nucleotide sequence that is complementary to nucleotides 510-532 of SEQ ID NO:
- 25.
Method for determining expression of a MAGE-C3 gene in a sample, comprising contacting said sample with (i) an oligonucleotide having a sequence set forth by nucleotides 175-195 of SEQ ID NO:
- 21 and (ii) an oligonucleotide having a sequence that is complementary to nucleotides 711-731 of SEQ ID NO:
- 21, under conditions favoring hybridization of the sequences of (i) or (ii) to an MAGE-C3 coding sequence, carrying out polymerase chain reaction and determining expression product to determine presence of an MAGE-C3 coding sequence in said sample.
Method for determining expression of a MAGE-B5 gene in a sample, comprising contacting said sample with (i) an oligonucleotide having a sequence set forth by nucleotides 370-394 of SEQ ID NO:
- 23 and (ii) an oligonucleotide having a sequence that is complementary to nucleotides 682-705 of SEQ ID NO:
- 23, under conditions favoring hybridization of the sequences of (i) or (ii) to an MAGE-B5 coding sequence, carrying out polymerase chain reaction and determining expression product to determine presence of an MAGE-B5 coding sequence in said sample.
Method for determining expression of a MAGE-B6 gene in a sample, comprising contacting said sample with (i) an oligonucleotide having a sequence set forth by nucleotides 114-137 of SEQ ID NO:
- 25 and (ii) an oligonucleotide having a sequence that is complementary to nucleotides 510-532 of SEQ ID NO:
- 25, under conditions favoring hybridization of the sequences of (i) or (ii) to an MAGE-B6 coding sequence, carrying out polymerase chain reaction and determining expression product to determine presence of an MAGE-B6 coding sequence in said sample.
Dependencies are inferred from the claim text.