Card domain containing polypeptides, encoding nucleic acids, and methods of use
AssigneeThe Burnham Institute
InventorsJohn C. Reed, Frederick F. Pio, Adam Godzik, Christian Stehlik, Jason S. Damiano, Sug Hyung Lee, Vasco A. Oliveira, Hideki Hayashi, Krzysztof Pawlowski
Life of this patent
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Abstract
The invention provides caspase recruitment domain (CARD)-containing polypeptides, CARD, NB-ARC, ANGIO-R, LRR and SAM domains therefrom, as well as encoding nucleic acid molecules and specific antibodies. The invention also provides related screening, diagnostic and therapeutic methods.
Claims30
An isolated nucleic acid molecule encoding a CARD-containing polypeptide, or a CARD, NB-ARC, ANGIO-R, LRR or SAM domain therefrom, selected from:
- (a) DNA encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NOS:
- 12, 168, 188, 170, 172, 174, 176, 97, 99, 101, 103, 178, 180, 182, 184, 86 and 90;
- and
- (b) DNA that hybridizes to the DNA of (a) under moderately stringent conditions, wherein said DNA encodes a biologically active polypeptide.
An isolated CARD-containing polypeptide, or a CARD, NB-ARC, ANGIO-R, LRR or SAM domain therefrom, comprising an amino acid sequence at least 70% identical to the amino acid sequence set forth in any of SEQ ID NOS:12, 168, 188, 170, 172, 174, 176, 97, 99, 101, 103, 178, 180, 182, 184, 86 and 90.
A composition comprising a compound selected from the group consisting of a CARD-containing polypeptide, a functional fragment therefrom, and an anti-CARD antibody;
- and a pharmaceutically acceptable carrier.
A chimeric polypeptide comprising a domain selected from the group consisting of SEQ ID NOS:168, 170, 172, 174, 176, 178, 180, 182 and 184.
A method of identifying an effective agent that modulates an activity of a NB-ARC domain of a CARD-containing polypeptide, comprising the steps of:
- (a) contacting a polypeptide comprising an NB-ARC domain set forth as either of SEQ ID NOS:174 or 180 with an agent known or suspected of modulating an activity of an NB-ARC domain;
- and
- (b) measuring the activity of the NB-ARC domain, whereby an increase or decrease of said activity identifies said agent as an agent that modulates the activity of the NB-ARC domain of said CARD-containing polypeptide;
- wherein the activity of the NB-ARC domain of said CARD-containing polypeptide is selected from homo-oligomerization, hetero-oligomerization, nucleotide hydrolysis, and nucleotide binding.
Dependencies are inferred from the claim text.