Antimicrobial mediated bacterial DNA delivery
OwnerDonata R. Sizemore, Jerald C. Sadoff +1inventors
InventorsDonata R. Sizemore, Jerald C. Sadoff, Jason C. Grove
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Abstract
This invention pertains in part to a method for delivering functional DNA or antigens. The desired DNA is introduced into attenuated bacteria able to enter cells. Once the bacteria is in the cell, antimicrobial agents are introduced such that they enter the mammalian cell and lyse the bacteria thereby allowing the delivery of carried functional DNA or antigens. The advantages of this method and its uses are described.
Claims10
A method for delivering functional DNA into a cell, said method comprising the steps of:
- (i) introducing said functional DNA into an attenuated bacteria able to enter cells;
- (ii) allowing said attenuated bacteria to enter cell;
- and
- (iii) lysing said bacteria inside said cell such that said DNA is released into said cell.
A method for delivering an antigen to a cell comprising:
- (i) introducing said antigen into an attenuated bacteria able to enter cells;
- (ii) allowing said attenuated bacteria to enter cell;
- and
- (iii) lysing said bacteria inside said cell such that said antigen is released into said cell.
A DNA delivery kit for the delivery of functional DNA into cells, said kit comprising attenuated Shigella containing said DNA, and an antimicrobial able to enter said cells and lyse said attenuated Shigella.
Dependencies are inferred from the claim text.