Functional expression of, and assay for, functional cellular receptors in vivo
AssigneeThe Trustees of Columbia University
InventorsStuart J. Firestein, Haiqing Zhao
Life of this patent
Estimating the term…
Abstract
Methods and materials for expressing and assaying functional neuronal receptors in neuronal cells, including methods for detecting particular odorant ligand specificity for particular odorant receptors and methods of using such. For example, methods and materials are provided for assaying for functional odor receptors in intact nasal epithelium of mammals such as rats and mice and for using such.
Claims31
A method for screening for cellular receptors having a particular receptor ligand specificity comprising the steps of:
- expressing at least one recombinantly produced cellular receptor in a cell located amongst intact tissue;
- contacting said recombinantly produced cellular receptor expressing cell with at least one candidate ligand;
- and
- assaying for functional interaction between said recombinantly produced cellular receptor and said candidate ligand.
A composition of matter comprising:
- a receptor-ligand complex of recombinantly produced odorant receptor I7 and a ligand selected from the group consisting of straight chain aldehydes having from 7 to 10 carbons in length.
A method for screening for neuronal receptors having a particular receptor ligand specificity comprising the steps of:
- expressing at least one recombinantly produced neuronal receptor in a neuronal cell located amongst intact tissue;
- isolating intact tissue expressing said recombinantly produced neuronal receptor;
- contacting said recombinantly produced neuronal receptor expressing cell with at least one candidate ligand;
- and
- assaying for functional interaction between said recombinantly produced neuronal receptor and said candidate ligand.
A method for screening for ligands having a particular cellular receptor specificity comprising the steps of:
- expressing at least one recombinantly produced cellular receptor in a cell located amongst intact tissue;
- contacting said recombinantly produced cellular receptor expressing cell with at least one candidate ligand;
- and
- assaying for functional interaction between said recombinantly produced cellular receptor and said candidate ligand.
Dependencies are inferred from the claim text.