Inactivation of microbes in biological fluids
AssigneePurePulse Technologies, Inc.
InventorsJeffrey Morse Holloway, Kenton Salisbury, William H. Cover, Mark Landers
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Abstract
The present invention relates to methods and treatment systems for inactivation of microbes and/or nucleic acids in biological fluids, especially platelet compositions without completely damaging antigens, enzymes and membrane functions. In accordance with the method of the invention, a biological fluid is illuminated with a light source having at least one wavelength within a range of 170 to 2600 nm to inactivate microbes in the composition and inactivate nucleic acids inside cells without destroying proteins (enzymes) and membrane functions.
Claims21
A method of inactivating microbes in a platelet composition, the method comprising illuminating the biological fluid with pulses of a light having at least one wavelength within a range of 170 to 2600 nm and a fluence greater than about 0.001 J/cm2, the illumination effective for inactivating microbes in the platelet composition and for decreasing platelet aggregation by not more than about 40%.
A method of inactivating microbes in a platelet composition, the method comprising illuminating the platelet solution with pulses of a light having at least one wavelength within a range of 170 to 2600 nm and a fluence level of about 0.05 to about 15 J/cm2, the illumination effective for inactivating microbes in the platelet composition by at least about 2 logs, and for decreasing platelet aggregation by not more than about 40%.
A method of inactivating microbes in a platelet composition, the method comprising:
- flowing the platelet composition through a treatment chamber, the treatment chamber being light transmissive to at least 1% of a light treatment having at least one wavelength within a range of 170 to 2600 nm;
- illuminating the platelet composition with the light as the platelet composition is flowed through the flexible treatment chamber;
- inactivating microbes within the platelet composition, the method effective for inactivating microbes in the platelet composition by at least about 2 logs, and for decreasing platelet aggregation by not more than about 40%.
A method for increasing shelf-life of a platelet composition, the method comprising:
- illuminating the platelet composition with pulses of a light having at least one wavelength within a range of 170 to 2600 nm and a fluence greater than about 0.001 J/cm2, and repeating the illumination of the platelet composition every 6 hours, the illumination effective for inactivating microbes and for providing a zero net increase of microbial counts in the platelet composition, wherein platelet aggregation is not decreased by more than about 40%.
A method for inactivating an endogenous nucleic acid strand, the method comprising illuminating an organisms containing the nucleic acid strand with at least one high-intensity, short duration pulse of incoherent polychromatic light in a broad spectrum.
A method for inactivating microbes in a platelet composition, the method comprising:
- illuminating the platelet composition with pulses of a light having at least one wavelength within a range of 170 to 2600 nm and a fluence greater than about 0.001 J/cm2, and repeating the illumination of the platelet composition every 6 hours, the illumination effective for inactivating microbes and for providing a zero net increase of microbial counts in the platelet composition,
- wherein platelet aggregation is not decreased by more than about 40%.
Dependencies are inferred from the claim text.