Method for recording and displaying fluorescence images with a high spatial resolution

A fluorescence microscope comprising:

• an optical illuminating system;
• an optical imaging system;
• an image recording system;
• and,
• a computer system for carrying out a method including the steps of:
• making several recordings of an image of an object sequentially at time intervals with an electronic camera to obtain partial images;
• introducing lateral displacements between said electronic camera and said object between said recordings;
• joining said partial images to form a compiled image of higher spatial resolution;
• obtaining signals of image points of said partial images at different time points;
• and,
• amplifying said signals with amplification factors which are dependent upon said time intervals between said recordings.

A computer system comprising:

• means for making several recordings of an image of an object sequentially at time intervals with an electronic camera to obtain partial images;
• means for introducing lateral displacements between said electronic camera and said object between said recordings;
• means for joining said partial images to form a compiled image of higher spatial resolution;
• means for obtaining signals of image points of said partial images at different time points;
• and,
• means for amplifying said signals with amplification factors which are dependent upon said time intervals between said recordings.

A computer program product which can be loaded into the memory of a digital computer, said computer program product comprising:

• a program having a software code for executing a method for recording and displaying fluorescence images with high spatial resolution, the method including the steps of:
• making several recordings of an image of an object sequentially at time intervals with an electronic camera to obtain partial images;
• introducing lateral displacements between said electronic camera and said object between said recordings;
• joining said partial images to form a compiled image of higher spatial resolution;
• obtaining signals of image points of said partial images at different time points;
• and,
• amplifying said signals with amplification factors which are dependent upon said time intervals between said recordings.