Mammalian cytokines; receptors; related reagents and methods
AssigneeSchering Corporation
InventorsPedro A. Reche-Gallardo, Vassilli Soumelis, Yong-Jun Liu, Rene de Waal Malefyt, J. Fernando Bazan, Robert A. Kastelein
Life of this patent
Estimating the term…
Abstract
Nucleic acids encoding mammalian cytokine receptor, e.g., for cytokine IL-B50, purified proteins and fragments thereof. Antibodies, both polyclonal and monoclonal, are also provided. Methods of using the compositions for both diagnostic and therapeutic utilities are described.
Claims21
A method of producing a ligand:receptor complex, comprising contacting:
- a) a substantially pure or recombinant mammalian IL-B50 with a receptor comprising the IL-7Rα or the Rδ2 subunit;
- b) a mammalian IL-B50 with a receptor comprising a substantially pure or recombinant IL-7Rα subunit;
- or
- c) a mammalian IL-B50 with a receptor comprising a substantially pure or recombinant Rδ2 subunit;
- thereby allowing said complex to form.
A method of modulating physiology or development of an IL-7Rα or Rδ2 expressing cell comprising contacting said cell to an exogenous agonist or antagonist of a mammalian IL-B50.
A method of modulating a signal to a cell mediated by IL-B50 comprising contacting said cell to an administered agonist or antagonist of IL-B50.
A method of selectively labeling a population of cells, said method comprising contacting said cells with an antibody which binds:
- a) IL-7Rα;
- b) Rδ2;
- or
- c) a complex comprising one of said subunits;
- thereby resulting in the identification of cells expressing said subunit or complex.
A method of testing a compound for ability to affect receptor-ligand interaction, said method comprising comparing the interaction of a receptor complex comprising IL-7Rα and/or Rδ2 subunit with IL-B50 in the presence and absence of said compound.
An isolated or recombinant protein complex comprising:
- a) at least 15 contiguous amino acid residues of SEQ ID NO:
- 2 and at least 15 contiguous amino acid residues of SEQ ID NO:
- 4;
- b) at least two distinct segments of at least 8 contiguous amino acid residues of SEQ ID NO:
- 2 and at least two distinct segments of at least 8 contiguous amino acid residues of SEQ ID NO:
- 4;
- or
- c) at least one segment at least 21 contiguous nucleotides of SEQ ID NO:
- 1 and at least one segment at least 21 contiguous nucleotides of SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.