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US 2002/0173620 A1Published application
Bifunctional fusion proteins formed from hirudin and TAP
OwnerPaul Habermanninventor
Life of this patent
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Abstract
The invention relates to bifunctional fusion proteins, which contain hirudin or a variant of hirudin and TAP (tick anticoagulant peptide) or a variant of TAP; the preparation and use of these bifunctional fusion proteins; and pharmaceutical compositions comprising these bifunctional fusion proteins.
Claims61
A bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP.
A DNA sequence coding for a bifunctional fusion protein selected from the group consisting of:
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein comprises the hirudin variant [Leu1, Thr2]-63-desulfatohirudin or a part of this hirudin variant,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein comprises the amino acids 1-63 from sequence SEQ ID NO:
- 15,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein TAP has the sequence SEQ ID NO:
- 17,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein the variant of TAP corresponds to a part of the sequence SEQ ID NO:
- 17,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein hirudin or its variant and TAP or its variant are connected via a spacer, and wherein said spacer consists of one or more amino acids, and
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein further comprises a signal peptide.
7 more elements
A process for the preparation of a bifunctional fusion protein comprising:
- incorporating a DNA sequence, which codes for the bifunctional fusion protein, into a cell, and
- expressing the DNA sequence in the cell,
- wherein said bifunctional fusion protein is selected from the group consisting of:
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein comprises the hirudin variant [Leu1, Thr2]-63-desulfatohirudin or a part of this hirudin variant,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein comprises the amino acids 1-63 from sequence SEQ ID NO:
- 15,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein TAP has the sequence SEQ ID NO:
- 17,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein the variant of TAP corresponds to a part of the sequence SEQ ID NO:
- 17,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP wherein hirudin or its variant and TAP or its variant are connected via a spacer, and wherein said spacer consists of one or more amino acids, and
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein further comprises a signal peptide.
10 more elements
A pharmaceutical composition comprising a bifunctional fusion protein, wherein said bifunctional fusion protein is selected from the group consisting of:
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein comprises the hirudin variant [Leu1, Thr2]-63-desulfatohirudin or a part of this hirudin variant,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein comprises the amino acids 1-63 from sequence SEQ ID NO:
- 15,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein TAP has the sequence SEQ ID NO:
- 17,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein the variant of TAP corresponds to a part of the sequence SEQ ID NO:
- 17,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein hirudin or its variant and TAP or its variant are connected via a spacer, and wherein said spacer consists of one or more amino acids, and
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein further comprises a signal peptide.
7 more elements
A nasal spray comprising a bifunctional fusion protein, wherein said bifunctional fusion protein is selected from the group consisting of:
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein comprises the hirudin variant [Leu1, Thr2]-63-desulfatohirudin or a part of this hirudin variant,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein comprises the amino acids 1-63 from sequence SEQ ID NO:
- 15,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein TAP has the sequence SEQ ID NO:
- 17,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein the variant of TAP corresponds to a part of the sequence SEQ ID NO:
- 17,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein hirudin or its variant and TAP or its variant are connected via a spacer, and wherein said spacer consists of one or more amino acids, and
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein further comprises a signal peptide.
7 more elements
A process for the production of a nasally administrable pharmaceutical composition comprising
- preparing a bifunctional fusion protein, and
- mixing said bifunctional fusion protein with a suitable pharmaceutical carrier,
- wherein said bifunctional fusion protein is selected from the group consisting of:
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein comprises the hirudin variant [Leu1, Thr2]-63-desulfatohirudin or a part of this hirudin variant,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein comprises the amino acids 1-63 from sequence SEQ ID NO:
- 15,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein TAP has the sequence SEQ ID NO:
- 17,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein the variant of TAP corresponds to a part of the sequence SEQ ID NO:
- 17,
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein hirudin or its variant and TAP or its variant are connected via a spacer, and wherein said spacer consists of one or more amino acids, and
- a bifunctional fusion protein formed from hirudin or a variant of hirudin and TAP or a variant of TAP, wherein said bifunctional fusion protein further comprises a signal peptide.
10 more elements
Dependencies are inferred from the claim text.