Modulation of pericyte proliferation
OwnerGeorge L. King, Susan Abrahamson +1inventors
InventorsGeorge L. King, Susan Abrahamson, Michael Pugsley
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Abstract
Methods and materials for modulating pericyte and epithelial cell proliferation relating to BPI protein products or inhibitors thereof are provided.
Claims39
A method of enhancing pericyte cell proliferation comprising administering to a subject in need thereof an amount of a BPI protein product effective to enhance proliferation of pericyte cells.
A method of inhibiting pericyte cell proliferation comprising administering to a subject in need thereof an effective amount of an agent that inhibits BPI protein product-induced proliferation of pericyte cells.
A method of enhancing retinal epithelial cell proliferation comprising administering to a subject in need thereof an amount of a BPI protein product effective to enhance proliferation of retinal epithelial cells.
A method of screening for a candidate inhibitor of BPI-induced proliferation of pericytes comprising the steps of:
- (a) detecting proliferation of pericytes in the presence of BPI protein product and in the presence and absence of a test compound;
- and
- (b) identifying said test compound as a candidate inhibitor of BPI-induced proliferation when proliferation of the pericytes is reduced in the presence of the test compound.
A method of screening a BPI protein product for the ability to enhance proliferation of pericytes comprising the steps of:
- (a) detecting proliferation of pericytes in the presence and absence of a BPI protein product;
- and
- (b) identifying a BPI protein product as a candidate enhancer of pericyte proliferation when proliferation of the pericytes is increased in the presence of the BPI protein product.
A method of screening for a candidate enhancer of pericyte proliferation comprising the steps of
- (a) measuring proliferation of pericytes in the presence and absence of a test compound;
- (b) measuring proliferation of pericytes in the presence of said test compound and a BPI protein product, said BPI protein product at a concentration effective to enhance pericyte proliferation;
- and
- (c) identifying said test compound as a candidate enhancer of pericyte proliferation when pericyte proliferation is increased in step (a) but not further increased in step (b).
A method of screening for a candidate enhancer of pericyte proliferation comprising the steps of:
- (a) measuring proliferation of pericytes in the presence and absence of a test compound;
- (b) measuring proliferation of pericytes in the presence of said test compound and a BPI protein product, said BPI protein product at a concentration effective to enhance pericyte proliferation;
- and
- (c) identifying said test compound as a candidate enhancer of pericyte proliferation when the increase in pericyte proliferation measured in step (a) is about the same as or less than the increase in pericyte proliferation measured in step (b).
A method of enhancing epithelial cell proliferation comprising administering to a subject in need thereof an amount of a BPI protein product effective to enhance proliferation of epithelial cells.
A method of screening for a candidate inhibitor of BPI-induced proliferation of epithelial cells comprising the steps of:
- (a) detecting proliferation of epithelial cells in the presence of BPI protein product and in the presence and absence of a test compound;
- and
- (b) identifying said test compound as a candidate inhibitor of BPI-induced proliferation when proliferation of the epithelial cells is reduced in the presence of the test compound.
A method of screening a BPI protein product for the ability to enhance proliferation of epithelial cells comprising the steps of:
- (a) detecting proliferation of epithelial cells in the presence and absence of a BPI protein product;
- and
- (b) identifying a BPI protein product as a candidate enhancer of epithelial cell proliferation when proliferation of the epithelial cells is increased in the presence of the BPI protein product.
A method of screening for a candidate enhancer of epithelial cell proliferation comprising the steps of:
- (a) measuring proliferation of epithelial cells in the presence and absence of a test compound;
- (b) measuring proliferation of epithelial cells in the presence of said test compound and a BPI protein product, said BPI protein product at a concentration effective to enhance epithelial cell proliferation;
- and
- (c) identifying said test compound as a candidate enhancer of epithelial cell proliferation when epithelial cell proliferation is increased in step (a) but not further increased in step (b).
A method of enhancing pericyte proliferation in a subject with diabetes-induced retinal vascular permeability comprising administering to the subject an amount of a BPI protein product effective to enhance proliferation of pericytes.
A method of enhancing pericyte proliferation in a subject suffering from a pericyte degeneration disorder that is complications of diabetes, diseases associated with the presence of autoantibodies to pericytes, age-related macular degeneration (ARMD), ovarian failure, multiple sclerosis, Alzheimer's disease, traumatic brain injury, a condition involving perturbation of the blood-brain-barrier, partial seizures, or and placental development in pregnancy, comprising administering to the subject an amount of a BPI protein product effective to enhance proliferation of pericytes.
A method of enhancing cell proliferation in a subject in need of wound healing, comprising administering to the subject an amount of a BPI protein product effective to enhance production of fibroblasts cells.
A method of enhancing cell proliferation in a subject suffering from a bone degenerative disorder, comprising administering to the subject an amount of a BPI protein product effective to enhance production of chondroblasts or osteoblasts.
A method for reducing pericyte proliferation in a subject suffering from a condition where pericyte proliferation is deleterious, that is hypertension, vascular calcifications, atherosclerotic plaques, atherosclerosis, restenosis, cerebrovascular ischemia, stroke, coronary artery disease, myocardial ischemia, myocardial infarction, peripheral vascular disease, Raynaud's syndrome, early occlusion of peripheral arteries and vascular remodeling associated with pulmonary hypertension, acute respiratory distress syndrome (ARDS), endometriosis or adenomyosis, comprising administering to the subject an amount of an agent that inhibits BPI protein product-induced proliferation of pericyte cells.
A method for enhancing cell proliferation in a subject suffering from a retinal epithelial degeneration disorder that is retinitis pigmentosa or age-related macular degeneration, comprising administering to the subject an amount of a BPI protein product effective to enhance proliferation of retinal epithelial cells.
Dependencies are inferred from the claim text.