Cyanide-free reagent, and method for detecting hemoglobin
OwnerEddine Merabet, Karina Shapiro +1inventors
InventorsEddine Merabet, Karina Shapiro, Melissa Cousino
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Abstract
A cyanide-free reagent for detecting hemoglobin is provided. The cyanide-free reagent includes a surfactant, a cyanide-free ligand selected from a nitrate, a nitrate salt, a nitrite, a nitrite salt, and combinations thereof, and a hydrogen ion concentration sufficient to maintain the pH of the reagent below about 9. A method and a kit for detecting hemoglobin in a blood sample using the cyanide-free reagent are also provided.
Claims28
A cyanide-free reagent for detecting hemoglobin comprising:
- a) a surfactant;
- b) a cyanide-free ligand selected from the group consisting of a nitrate, a nitrate salt, a nitrite, a nitrite salt, and combinations thereof;
- and
- c) a hydrogen ion concentration sufficient to maintain the pH of the reagent below about 9
A method for detecting hemoglobin in a blood sample comprising the steps of:
- a) combining a blood sample with a cyanide-free reagent comprising:
- i) a surfactant,
- ii) a cyanide-free ligand selected from the group consisting of a nitrate, a nitrate salt, a nitrite, a nitrite salt, and combinations thereof, and
- ii) a hydrogen ion concentration sufficient to maintain the pH of the reagent below about 9;
- and
- b) measuring the absorbance of a chromogen formed by reaction of the ligand with the heme in the blood sample.
A kit for detecting hemoglobin in a blood sample comprising the component parts of:
- a) a cyanide-free reagent consisting of:
- a) a cyanide-free reagent consisting of:
- i) a surfactant,
- ii) a cyanide-free ligand selected from the group consisting of a nitrate, a nitrate salt, a nitrite, a nitrite salt, and combinations thereof, and
- iii) a hydrogen ion concentration sufficient to maintain the pH of the reagent below about 9.
Dependencies are inferred from the claim text.