Cell line and method of making and using same
OwnerCharleata A. Carterinventor
Life of this patent
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Abstract
A cell line isolated from a patient with metastatic adenocarcinoma consistent with primary endometrial cancer. These cells (“CAC-1 cells”) originated from glandular epithelial cells forming a primary tumor of the endometrium of the uterus. In general, these cells are poorly differentiated and highly invasive. Besides manifestations of abnormal karyotype, another distinguishing characteristic may be that typical CAC-1 cells are hyperdiploid, having 48 chromosomes rather than the standard 46 chromosomes; they are triploid at chromosomes 3, 7 and 17, but only haploid at chromosome 14.
Claims20
A cellular composition, comprising cells isolated from a poorly differentiated uterine cancer.
A line of cells originating from a specimen of human endometrial adenocarcinoma wherein a plurality of said cells behaves in substantially equivalent ways at the morphological, physiological or molecular levels as a cell of said sample.
A method of culturing cells in vitro, comprising the steps introducing said cells to media comprising a 1:1 mixture of Medium 199 and Ham's F12 supplemented with up to 4% serum, antibiotics, anti-micotics and growth factors;
- and culturing said cells under conditions for proliferation.
Dependencies are inferred from the claim text.