Method and means for producing high titer, safe, recombinant lentivirus vectors
AssigneeCell Genesys, Inc.
InventorsLuigi Naldini, Thomas Dull, Deborah A. Farson, Rochelle Witt
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Abstract
Lentiviral vectors modified at the 5′ LTR or both the 5′ and 3′ LTR's are useful in the production of recombinant lentivirus vectors. Such vectors can be produced in the absence of a functional tat gene. Multiple transformation of the host cell with the vector carrying the transgene enhances virus production.
Claims20
A lenitiviral packaging system comprising a structural lentiviral vector system comprising a first lentiviral vector that encodes a structural gene selected from a gag gene, a pol gene or both gag and pol genes, wherein the lentiviral packaging system lacks a functional tat gene.
The lentiviral packaging system 7, wherein the heterologous regulatory element comprises a CMV promoter.
Dependencies are inferred from the claim text.