Secreted salivary zsig63 polypeptide
OwnerDavid A. Adler, Paul O. Sheppardinventors
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Abstract
The present invention relates to polynucleotide and polypeptide molecules for zsig63, a novel secreted salivary protein. The polypeptides, and polynucleotides encoding them, may exhibit anti-microbial activity and may be used in the study or treatment of microbial infections. The polynucleotides encoding zsig63, are located on chromosome 4, and can be used to identify a region of the genome associated with human disease states. The present invention also includes antibodies to the zsig63 polypeptides.
Claims18
An isolated polynucleotide encoding a zsig63 polypeptide comprising a sequence of amino acid residues that is at least 90% identical to an amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence as shown in SEQ ID NO:
- 2 from amino acid number 16 (Arg) to amino acid number 37 (Ser);
- (b) the amino acid sequence as shown in SEQ ID NO:
- 2 from amino acid number 38 (Leu) to amino acid number 126 (Ala);
- (c) the amino acid sequence as shown in SEQ ID NO:
- 2 from amino acid number 127 (Pro) to amino acid number 219 (Gln);
- (d) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 16 (Arg) to amino acid number 219 (Gln);
- and
- (e) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 1 (Met) to amino acid number 219 (Gln).
An expression vector comprising the following operably linked elements:
- a transcription promoter;
- a DNA segment encoding a zsig63 polypeptide comprising an amino acid sequence that is at least 90% identical to the amino acid sequence shown in SEQ ID NO:2 from amino acid number 16 (Arg) to amino acid number 219 (Gln);
- and
- a transcription terminator.
A DNA construct encoding a fusion protein, the DNA construct comprising:
- a first DNA segment encoding a polypeptide selected from the group consisting of:
- (a) the amino acid sequence of SEQ ID NO:
- 2 from residue number 1 (Met) to residue number 15 (Ala);
- (b) the amino acid sequence of SEQ ID NO:
- 2 from residue number 16 (Arg) to residue number 37 (Ser);
- (c) the amino acid sequence of SEQ ID NO:
- 2 from residue number 38 (Leu) to residue number 126 (Ala);
- (d) the amino acid sequence of SEQ ID NO:
- 2 from residue number 127 (Pro) to residue number 219 (Gin);
- and
- (e) the amino acid sequence of SEQ ID NO:2 from residue number 16 (Arg) to residue number 219 (Gln);
- mad
- at least one other DNA segment encoding an additional polypeptide,
- wherein the first and other DNA segments are connected in-frame;
- and
- encode the fusion protein.
An isolated zsig63 polypeptide comprising a sequence of amino acid residues that is at least 90% identical to an amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence as shown in SEQ ID NO:
- 2 from amino acid number 16 (Arg) to amino acid number 37 (Ser);
- (b) the amino acid sequence as shown in SEQ ID NO:
- 2 from amino acid number 38 (Leu) to amino acid number 126 (Ala);
- (c) the amino acid sequence as shown in SEQ ID NO:
- 2 from amino acid number 127 (Pro) to amino acid number 219 (Gln);
- (d) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 16 (Arg) to amino acid number 219 (Gln);
- and
- (e) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 1 (Met) to amino acid number 219 (Gln).
A method of detecting, in a test sample, the presence of an agonist of zsig63 protein activity, comprising:
- transfecting a zsig63-responsive cell, with a reporter gene construct that is responsive to a zsig63-stimulated cellular pathway;
- and
- adding a test sample;
- and
- comparing levels of response in the presence and absence of the test sample, by a biological or biochemical assay;
- and
- determining from the comparison, the presence of the agonist of zsig63 activity in the test sample.
Dependencies are inferred from the claim text.