Nucleic acid sequences encoding type III tenebrio antifreeze proteins and method for assaying activity

A thermal hysteresis protein derived from the Tenebrionoidea Superfamily which lowers the freezing point of a solution without effecting the melting point of the solution.

A consensus sequence with a nucleotide sequence from the group consisting of SEQ ID NO.'s 44 to 47.

A consensus sequence with an amino acid sequence from the group consisting of SEQ ID NO.'s 44 to 47.

A consensus sequence with amino acid sequences as in SEQ ID NO. 48.

A primer having a nucleotide sequence selected from the group consisting of SEQ ID NO.'s 40 to 43.

A method for producing a polypeptide having antifreeze properties comprising:

• forming a cloning vector with a Tm 12.86 family member gene encoding an antifreeze polypeptide;
• transferring genes of said cloning vector into DNA of host cell to create a transformed cell;
• expressing a mRNA sequence and a translated amino acid sequence from said recombinant expression vector, said sequence being isoforms of said Tm 12.86 T.molitor antifreeze polypeptide.

A method for providing antifreeze or recrystallization inhibition properties to a subject formulation comprising incorporating at least 0.1 micro gm to about 1 mg of an activated polypeptide into 1 mL of a subject formulation to obtain recrystallization inhibition or about 1 mg to about 25 mg of said activated polypeptide into about 1 mL of a subject formulation to thermal hysteresis.

Native endogenous Type III anti-freeze proteins from the Tenebrionoidea Superfamily.

A Tm 12.86 antibody/antiserum which is used as a screening device to identify positive recombinant plaques containing cloned inserts capable in an expression vector system to produce recombinant products recognized by the antibody/antiserum.

A Tm 12.86 antibody/antiserum which is used as a screening device to screen cDNA libraries in an expression system, including cross-species cDNA libraries to identify homologous sequences in other species.