Process for producing microbial transglutaminase
AssigneeAjinomoto Co., Inc.
InventorsKeiichi Yokoyama, Nami Nakamura, Tetsuya Miwa, Katsuya Seguro
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Abstract
Disclosed are a protein having a transglutaminase activity, which comprises a sequence ranging from serine residue at the second position to proline residue at the 331st position in an amino acid sequence represented by SEQ ID No. 1 wherein the N-terminal amino acid of the protein corresponds to serine residue at the second position of SEQ ID No. 1, a DNA encoding the protein, a transformant having the DNA, and a process for producing a protein having a transglutaminase activity, which comprises the steps of culturing the transformant in a medium. The protein can be produced in a large amount with the transformant using a host such as E. coli.
Claims25
A protein having a transglutaminase activity, which comprises a sequence ranging from serine residue at the second position to proline residue at the 331st position in an amino acid sequence represented by SEQ ID No. 1 wherein the N-terminal amino acid of the protein corresponds to serine residue at the second position of SEQ ID No. 1.
A DNA comprising a sequence ranging from thymine base at the fourth position to guanine base at the 993rd position in the base sequence of SEQ ID No. 2.
A DNA consisting of a sequence ranging from thymine base at the fourth position to guanine base at the 993rd position in the base sequence of SEQ ID No. 2.
Dependencies are inferred from the claim text.