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US 2002/0173016 A1Published application
High-throughput nucleic acid polymerase devices and methods for their use
OwnerHelmut Wurst, Ningyue Zhaoinventors
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OwnerHelmut Wurst, Ningyue Zhaoinventors
Estimating the term…
High throughput lyophilized polymerase devices and methods for their use in the production of nucleic acids using template dependent polymerase reactions are provided. The subject devices are typically made up of a multi-well substrate that includes in a least one well a lyophilized nucleic acid polymerase composition. The subject nucleic acid polymerase compositions include at least one polymerase and a carbohydrate stabilizing composition that is made up of at least one low molecular weight sugar and a starch. In many embodiments, the compositions also include buffer components and nucleotides, as well as a temperature dependent polymerase inhibitor, e.g., a polymerase specific antibody. Also provided are kits that include the subject devices.
A substrate having at least one well filled with a lyophilized nucleic acid polymerase composition comprising:
A lyophilized polymerase composition comprising:
A method of producing a nucleic acid using a template dependent nucleic acid polymerase reaction, said method comprising:
A kit for use in a method of producing a nucleic acid using a template dependent nucleic acid polymerase reaction, said kit comprising:
Dependencies are inferred from the claim text.