Methods and materials relating to novel CD39-like polypeptides
AssigneeHyseq, Inc.
InventorsJohn Ford, Julio J. Mulero, George Yeung
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Abstract
The invention provides novel polynucleotides isolated from cDNA libraries of human fetal liver-spleen and macrophage as well as polypeptides encoded by these polynucleotides and mutants or variants thereof. The polypeptides correspond to a novel human CD39-like protein. Other aspects of the invention include vectors containing polynucleotides of the invention, and related host cells as well a processes for producing novel CD39-like polypeptides, and antibodies specific for such polypeptides.
Claims45
An isolated polynucleotide encoding an apyrase and/or NDPase and comprising a nucleotide sequence having at least about 80% sequence identity to a human polynucleotide selected from the group consisting of:
- (a) a polynucleotide having the nucleotide sequence of SEQ ID NO. 2;
- and
- (b) a polynucleotide having the protein coding nucleotide sequence of the polynucleotide sequence of (a).
An isolated polynucleotide encoding an apyrase and comprising a nucleotide sequence having at least about 90% sequence identity to a polynucleotide selected from the group consisting of:
- (a) a polynucleotide having the nucleotide sequence of SEQ ID NO. 2;
- and
- (b) a polynucleotide having the protein coding nucleotide sequence of the polynucleotide sequence of (a).
An isolated polynucleotide encoding a polypeptide with apyrase and/or NDPase activity, comprising a polynucleotide selected from the group consisting of:
- (a) polynucleotides that encode the mature protein coding amino acid sequence of SEQ ID NO. 3.
An isolated polynucleotide encoding a polypeptide with apyrase and/or NDPase activity that hybridizes under stringent conditions to the complement of a polynucleotide of SEQ ID NO. 2.
An isolated human polypeptide with apyrase and/or NDPase activity comprising:
- (a) the mature protein coding sequence of SEQ ID NO. 3;
- or
- (b) an amino acid sequence having at least about 80% sequence identity to SEQ ID NO. 3.
An isolated polypeptide with apyrase and/or NDPase activity comprising:
- (a) the CD39-like protein coding sequence of SEQ ID NO. 3;
- or
- (b) an amino acid sequence having at least about 90% sequence identity to SEQ ID NO. 3.
A chimeric polypeptide comprising one or more domains of a CD39-like polypeptide fused to one or more domains of heterologous peptide or polypeptide, e.g., an immunoglobulin constant region.
A method of inhibiting platelet aggregation in a mammalian subject comprising the step of reducing the ratio of ADP:ATP in said mammalian subject to a less than normal ratio.
A method of identifying a compound capable of reducing the ratio of ADP:ATP to a less than normal ratio comprising the steps of:
- (a) determining apyrase activity of said compound on ATP;
- (b) determining apyrase activity of said compounds on ADP;
- and
- (c) selecting a compound that has greater activity with respect to ADP compared to ATP.
Dependencies are inferred from the claim text.