Composition for qualitative screening of phosphate solubilizing microorganisms and a qualitative method for screening microorganisms
AssigneeCouncil of Scientific and Industrial Research
InventorsChandra Shekhar Nautiyal, Sangeeta Mehta, Palpu Pushpangadan
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Abstract
The present invention relates to a composition useful for qualitative screening of phosphate solubilizing microorganism, and also relates to a synergistic composition for qualitative screening of phosphate solubilizing microorganisms, based upon visual observation.
Claims8
A synergistic growth medium composition for fast screening of phosphate solubilizing microorganisms,comprising:
- 4 Glucose 5 to 30 gm/Lt.;
- Ca3 (PO4)2 1 to 20 gm/Lt.;
- MgCl2.6H2O 0.5 to 20 gm/Lt.;
- MgSO4.7H2O 0.05 to 5 gm/Lt.;
- KCl 0.025 to 5 gm/Lt.;
- (NH4)2SO4 0.01 to 2 gm/Lt.;
- and BPB 0.01 to 0.1 gm/Lt.
A composition as claimed in claim 1 for the screening of phosphate solubilizing microorganisms comprising:
- 5 Glucose 10 gm/Lt.;
- Ca3 (PO4)2 5 gm/Lt.;
- MgCl2.6H2O 5 gm/Lt.;
- MgSO4.7H2O 0.25 gm/Lt.;
- KCl 0.2 gm/Lt.;
- (NH4)2SO4 0.1 gm/Lt.;
- and BPB 0.025 gm/Lt.
A composition as claimed in claim 1 wherein, the ingredients used in the composition are of commercial grade.
A composition as claimed in claim 1 is used for qualitative analysis of bacteria from soil, effect of various carbon, nitrogen, salt and phosphate solubilization—defective transposon mutants.
A qualitative method for easy and fast screening of phosphate solubilizing comprising a synergistic growth medium composition containing essential bromophenol blue (BPB) in the range of 0.01 to 0.1 gm/Lt.
A kit for the screening of phosphate solubilizing microorganisms comprising BPB in a growth medium, said kit comprising:
- 8 Glucose 10 gm/Lt.;
- Ca3 (PO4)2 5 gm/Lt.;
- MgCl2.6H2O 5 gm/Lt.;
- MgSO4.7H2O 0.25 gm/Lt.;
- KCl 0.2 gm/Lt.;
- (NH4)2SO4 0.1 gm/Lt.;
- and BPG 0.025 gm/Lt.
Dependencies are inferred from the claim text.