Nucleotide sequences
OwnerJan Horling, Magnus Jansson +4inventors
InventorsJan Horling, Magnus Jansson, Eva Danielsson, Per Johansson, Staffan Lake, Joakim Nilsson
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Abstract
The present invention relates to human metalloprotease M8 polypeptides and to nucleic acid molecules coding for such polypeptides. The invention further relates to vectors and cells comprising the nucleic acid molecules, as well as to methods for screening for test compounds which affect the insulin-signaling pathway.
Claims35
An isolated nucleic acid molecule comprising a nucleotide sequence that is at least 85% identical to the sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, or SEQ ID NO:7.
An isolated nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence that is at least 85% identical to the sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8.
An isolated nucleic acid comprising a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8.
An isolated nucleic acid molecule comprising a nucleotide sequence that hybridizes under stringent hybridization conditions to the sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, or a complete complement thereof.
An isolated nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide comprising at least 100 contiguous amino acid residues of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8.
An isolated nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide comprising an immunogenic fragment of at least 20 amino acids of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8.
A substantially pure polypeptide comprising an amino acid sequence that is at least 85% identical to the sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8.
A substantially pure polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8.
A substantially pure polypeptide comprising at least 100 contiguous amino acid residues of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8.
A substantially pure polypeptide comprising an immunogenic fragment of at least 20 amino acids of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8.
A method of identifying an agent that binds to a metalloprotease M8, the method comprising:
- contacting a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8 with a candidate agent;
- and
- determining that the candidate agent binds to the polypeptide,
- to thereby identify a candidate agent that binds to a metalloprotease M8.
A method for disrupting a metalloprotease M8 gene in a non-human embryonic stem cell, the method comprising:
- providing a nucleotide sequence capable of disrupting a metalloprotease M8 gene;
- and
- introducing the nucleotide sequence into a non-human embryonic stem-cell under conditions such that nucleotide sequence is homologously recombined into the metalloprotease M8 gene in the genome of the cell, to produce a cell containing at least one disrupted metalloprotease M8 allele.
Dependencies are inferred from the claim text.