Method of screening for antimicrobial compounds
AssigneeSmithKline Beecham Corporation
InventorsKenneth Coleman, Ian A. Critchley
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Abstract
The present invention provides a method for screening for antimicrobial compounds using in a whole cell assay using bacteria, particularly bacteria of the genus Staphylococcus and Streptococcus.
Claims14
A method of screening for bacteriostatic and bactericidal compounds comprising the steps of:
- (a) providing a composition comprising at least two different bacteria each of said bacteria comprising a marker gene the product of which is detectable at different wavelengths of the light spectrum;
- (b) contacting said composition of step (a) with a test compound;
- (c) detecting whether a change in intensity of least one of said different wavelengths occurs;
- and
- (d) determining whether said test compound correlates with said change in intensity.
A method of screening for bacteriostatic and bactericidal compounds comprising the steps of:
- (a) providing a composition comprising at least two different bacteria each comprising a compound which is detectable at different wavelengths of the light spectrum;
- (b) contacting said composition of step (a) with a test compound;
- (c) detecting whether a change in intensity of least one of said different wavelengths occurs;
- and
- (d) determining whether said test compound correlates with said change in intensity.
A method of screening for bacteriostatic and bactericidal compounds comprising the steps of:
- (a) providing a composition comprising at least two different bacteria each comprising a compound which is detectable at a wavelengths of the light spectrum;
- (b) contacting said composition of step (a) with a test compound;
- (c) detecting whether a change in intensity of least one of said wavelengths occurs;
- and
- (d) - determining whether said test compound correlates with said change in intensity.
A composition comprising at least two isolated cultures of bacteria wherein at least two of said bacteria is selected from the group consisting of:
- Gram positive organisms, Streptococcus, Staphylococcus, Gram negative organisms, E. coli, K. pneumoniae, and Legionella pneumophila.
A composition comprising at least two cultures of bacteria wherein at least two of said bacteria comprises at least one marker genes selected from the group consisting of lux, luxCDABE, luxAB and luc.
A kit comprising at least two isolated cultures of bacteria wherein at least two of said bacteria is selected from the group consisting of:
- Gram positive organisms, Streptococcus, Staphylococcus, Gram negative organisms, E. coli, K. pneumoniae, and Legionella pneumophila.
A kit comprising at least two cultures of bacteria wherein at least two of said bacteria comprises at least one marker genes selected from the group consisting of lux, luxCDABE, luxAB and luc.
Dependencies are inferred from the claim text.