Biochips comprising nucleic acid/protein conjugates
OwnerHongxiang Liu, Bassil I. Dahiyat +1inventors
InventorsHongxiang Liu, Bassil I. Dahiyat, Min Li
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Abstract
The present invention is directed to the formation of protein arrays through the use of nucleic acid/protein (NAP) conjugates, which allow the covalent attachment of proteins and the nucleic acids encoding them. By using vectors that include capture sequences that will hybridize to capture probes on a nucleic acid array, the NAP conjugates including the proteins of interest are arrayed and used in a wide variety of applications.
Claims16
A composition comprising a substrate comprising an array of capture probes, a plurality of which are hybridized to an expression vector comprising:
- a) a fusion nucleic acid comprising:
- i) nucleic acid encoding said NAM enzyme;
- and
- ii) nucleic acid encoding said candidate protein;
- b) a capture sequence;
- and
- c) an enzyme attachment sequence (EAS);
- wherein said EAS and said NAM enzyme are covalently attached.
A composition comprising a substrate comprising an array of capture probes, a plurality of which are hybridized to a nucleic acid/protein (NAP) conjugate comprising:
- a) a fusion polypeptide comprising:
- i) a Rep protein;
- and
- ii) a candidate protein;
- b) an expression vector comprising:
- i) a fusion nucleic acid comprising:
- 1) nucleic acid encoding said Rep protein;
- and
- 2) nucleic acid encoding said candidate protein;
- ii) a capture sequence;
- and
- iii) an enzyme attachment sequence (EAS);
- wherein said EAS and said Rep protein are covalently attached.
A method of detecting the presence of a target analyte in a sample comprising:
- a) contacting said sample with a biochip comprising:
- i) a substrate comprising an array of capture probes, a plurality of which are hybridized to a nucleic acid/protein (NAP) conjugate each comprising:
- 1) a fusion polypeptide comprising:
- A) a Rep protein;
- and
- B) a candidate protein;
- 2) an expression vector comprising:
- A) a fusion nucleic acid comprising:
- i) nucleic acid encoding said Rep protein;
- and
- ii) nucleic acid encoding said candidate protein;
- B) a capture sequence;
- and
- C) an enzyme attachment sequence (EAS);
- wherein said EAS and said Rep protein are covalently attached, under conditions wherein said target analyte can bind to at least one of said candidate proteins to form an assay complex;
- and
- b) detecting the presence of said target analyte on said substrate.
A method of making biochips comprising:
- a) providing a substrate comprising an array of capture probes, a plurality of which are hybridized to a plurality of expression vectors each comprising:
- i) a fusion nucleic acid comprising:
- 1) a nucleic acid encoding a NAM enzyme;
- and
- 2) a nucleic acid encoding a different candidate protein;
- ii) a capture sequence;
- and
- iii) an enzyme attachment sequence (EAS);
- wherein said EAS and said NAM enzyme are covalently attached and wherein said capture sequences are hybridized to said capture probes;
- b) adding an in vitro expression system to form fusion polypeptides, each comprising:
- i) said (NAM) enzyme;
- and
- ii) said different candidate protein;
- c) forming a plurality of NAP conjugates on the surface of said biochip;
- wherein said NAP conjugates are covalently linked to said EAS of said expression vector.
A method of making NAP conjugates comprising:
- a) providing a substrate comprising an array of capture probes, a plurality of which are hybridized to a plurality of expression vectors each comprising:
- i) a fusion nucleic acid comprising:
- 1) a nucleic acid encoding a NAM enzyme;
- and
- 2) a nucleic acid encoding a different candidate protein;
- ii) a capture sequence;
- and
- iii) an enzyme attachment sequence (EAS);
- wherein said EAS and said NAM enzyme are covalently attached and wherein said capture sequences are hybridized to said capture probes;
- b) contacting said substrate with an in vitro expression system to form a plurality of NAP conjugates each comprising:
- i) said NAM enzyme;
- ii) said different candidate protein;
- and
- iii) at least a portion of said expression vector.
A composition comprising a substrate comprising an array of capture probes, a plurality of which are hybridized to:
- a) a plurality of expression vectors each comprising:
- 1) a fusion nucleic acid comprising:
- i) nucleic acid encoding said NAM enzyme;
- and
- ii) nucleic acid encoding said candidate protein;
- 2) a capture sequence;
- and
- 3) an enzyme attachment sequence (EAS);
- wherein said EAS and said NAM enzyme are covalently attached;
- and,
- b) a plurality of nucleic acid/protein (NAP) conjugates comprising:
- 1) a fusion polypeptide comprising:
- i) a Rep protein;
- and
- ii) a candidate protein;
- 2) an expression vector comprising:
- i) a fusion nucleic acid comprising:
- 1) nucleic acid encoding said Rep protein;
- and
- 2) nucleic acid encoding said candidate protein;
- ii) a capture sequence;
- and
- iii) an enzyme attachment sequence (EAS);
- wherein said EAS and said Rep protein are covalently attached.
Dependencies are inferred from the claim text.