Method for isolating DNA
AssigneeBio 101
InventorsJames W. Gautsch, Michael G. Saghbini, David A. Lippman, Richard C. Dana
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Abstract
The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.
Claims29
A method of isolating deoxyribonucleic acid (DNA) from a biological material which comprises mechanically releasing said DNA from said material by the application of rapidly oscillating reciprocal mechanical energy to said material in the presence of a preselected volume of a liquid medium in a container to produce a released DNA solution, said application of said energy conducted by subjecting said material to oscillations at an oscillatory rate of between about 25 hertz (Hz) to about 133 Hz and effective to produce an average linear acceleration in the material in the range of from about 150 times gravity (g) to about 415 times g for a period of time of between about 3 seconds to about 5 minutes.
Dependencies are inferred from the claim text.