Method and system for nucleic acid sequencing
OwnerMark W. Perlininventor
Life of this patent
Estimating the term…
Abstract
A method of nucleic acid sequencing comprising the steps (a) amplifying a nucleic acid sample to produce an amplified DNA product; (b) extending a sequencing primer bound to the DNA product in the presence of terminating nucleotide analogs to produce a collection of labeled nucleic acid products; (c) detecting a total amount of label present in the collection to produce a measurement; and (d) combining a plurality of measurements to determine DNA sequence information about the sample. A system for nucleic acid sequencing which uses terminating nucleotide analogs to quantitatively determine fragment length and sequence information.
Claims16
A method of nucleic acid sequencing comprising the steps:
- (a) amplifying a nucleic acid sample to produce an amplified DNA product;
- (b) extending a sequencing primer bound to the DNA product in the presence of terminating nucleotide analogs to produce a collection of labeled nucleic acid products;
- (c) detecting a total amount of label present in the collection to produce a measurement;
- and
- (d) combining a plurality of measurements to determine DNA sequence information about the sample.
A system for nucleic acid sequencing comprising:
- (a) a means for amplifying a nucleic acid sample to produce an amplified nucleic acid product;
- (b) a means for extending a sequencing primer bound to the DNA product in the presence of terminating nucleotide analogs to produce a collection of labeled nucleic acid products, said extending means in connection with the amplified product;
- (c) a means for detecting a total amount of label present in the collection to produce a measurement, said detecting means in connection with the collection;
- and
- (d) a means for combining a plurality of measurements to determine DNA sequence information about the sample, said combining means in connection with the measurement.
A method for obtaining information about a signal comprising the steps:
- (a) inducing a decay function;
- (b) imposing the decay function on a signal;
- (c) forming a numerical quantity that characterizes the signal's behavior in the presence of the decay function;
- (d) combining a plurity of such numerical quantities to obtain information about the signal.
Dependencies are inferred from the claim text.