Protein activity screening of clones having dna from uncultivated microorganisms
AssigneeDiversa Corporation
InventorsJAY M. SHORT, BARRY MARRS, JAY M. STEIN
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Abstract
Disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein, e.g. enzyme, activity by screening for a specified protein, e.g. enzyme, activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism; and (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified protein, e.g. enzyme, activity.
Claims10
A process of screening clones having DNA from an uncultivated microorganism for a specified small molecule activity, which process comprises:
- (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism;
- and
- (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified small molecule activity.
A process of screening clones having DNA from an uncultivated microorganism for a specified enzyme activity, which process comprises:
- screening for a specified enzyme activity in a library of clones prepared by
- (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism;
- and
- (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified enzyme activity.
Dependencies are inferred from the claim text.