Biologically active chloroform fraction of an extract obtained from a mangrove plant Salvadora persica L
AssigneeCouncil of Scientific and Industrial Research
InventorsUsha Goswami, Nazarine Fernandes
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Abstract
The invention discloses a process of extracting, fractionating and purifying bioactive molecules from an associated mangrove plant, methods of screening for pharmacological activities of crude extract, its fractions and purified compounds and use of the chloroform fraction of the crude extract as anti-spasmodic, anti-arrhythmic and anti-cholinergic agent.
Claims32
A process for the extraction and purification of a biologically active extract from the plant Salvadora persica, comprising a steps of
- i) collecting and processing the plant parts of Salvadora persica,
- ii) preparing a crude extract from the plant parts of Salvadora persica,
- iii) testing the crude extract using methods of pharmacology,
- iv) fractionating the crude extract,
- v) testing the fractions using methods of pharmacology,
- vi) isolating the pure compounds by column chromatography,
- vii) testing the pure compounds by using methods of pharmacology, and
- viii) identifying the compounds by spectroscopy.
A Pharmaceutical composition exhibiting anti-cholinergic, anti-spasmodic, and anti-arrhythmic activity, said composition comprising the chloroform fraction of the extract obtained from the plant Salvadora persica optionally, with conventional additives.
A method for the treatment of arrhythmias, said method comprising the step of administering a therapeutically effective amount of extract obtained from the plant Salvadora persica, optionally with conventional additives to a subject afflicted with arrhythmias.
A method for the treatment of spasmodic conditions such as muscular spasms, said method comprising the step of administering a therapeutically effective amount of extract obtained from the plant Salvadora persica, optionally with conventional additives to a subject in need thereof.
A method for the treatment of cholinergic conditions such as bronchial asthma, said method comprising the step of administering a therapeutically effective amount of extract obtained from the plant Salvadora persica, optionally with conventional additives to a subject in need thereof.
Dependencies are inferred from the claim text.