Process for preparing an autologous platelet gel and membrane thereof, and a kit for carrying out this process
AssigneeMaria Cristina Sacchi
InventorsMaria Cristina Sacchi, Marco Bellanda
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Abstract
A process is disclosed for preparing an autologous platelet gel and membranes thereof comprising mixing a platelet concentrate with a calcium salt and batroxobin. This process encompassing the use of batroxobin as the gel activator allows to overcome the prior art processes drawbacks connected with the use for the same purpose of human of bovine thrombin. A kit is also described for carrying out this process.
Claims15
A process for preparing a platelet gel comprising mixing a platelet concentrate with calcium chloride and batroxobin, wherein said platelet concentrate is obtained with a process comprising:
- i) centrifuging 40-50 ml of venous blood at 180 g for 20 minutes thereby obtaining two phases a dark one constituted by precipitated red and white cells at the bottom of the centrifuge probe , and a clear one visible in the upper part of the same tube, consisting of platelet rich plasma
- ii) centrifuging the recovered platelet rich plasma at 580 g for 20 minutes thereby obtaining platelets sedimentation as “pellets” whereas the liquid supematant consists of platelet poor plasma,
- iii) suspending said pellets in an aliquot of said platelet poor plasma necessary to obtain a platelet concentrate of about 6 ml.
A process for preparing a platelet gel comprising mixing a platelet concentrate with an organic or inorganic calcium salt and batroxobin, wherein said platelet concentrate is obtained with a process comprising:
- i) centrifuging 40-50 ml of venous blood at 180 g for 20 minutes thereby obtaining two phases a dark one constituted by precipitated red and white cells at the bottom of the centrifuge probe, and a clear one visible in the upper part of the same tube, consisting of platelet rich plasma
- ii) centrifuging the recovered platelet rich plasma at 580 g for 20 minutes thereby obtaining platelets sedimentation as “pellets” whereas the liquid supematant consists of platelet poor plasma,
- iii) suspending said pellets in an aliquot of said platelet poor plasma necessary to obtain a platelet concentrate of about 6 ml.
The kit according to claim 12, wherein the capacity of the capsule (3) is comprised between 10 and 20 ml.
Dependencies are inferred from the claim text.